在MdMYB109和MdHXK1基因变异之间的表观作用对果果中的糖含量有很大影响
Zhongyan Zhang1, Zhenyu Huang2, Bei Wu1
1College of Horticulture, China Agricultural University, Beijing, 100193, China.
The Plant journal : for cell and molecular biology
|December 9, 2024
概括
鉴定出了果果糖含量的遗传变异. 关键基因MdMYB109和MdHXK1调节糖分水平,改善了果育种的基因组学辅助预测模型.
科学领域:
- 植物遗传学 植物遗传学
- 分子生物学分子生物学
- 农业科学 农业科学
背景情况:
- 果糖含量等定量特征由许多具有小影响的基因控制,使遗传网络分析和标记器辅助育种复杂化.
- 果果的可溶糖含量是一个复杂的特征,受到许多定量特征位置 (QTL) 的影响.
研究的目的:
- 为了确定果中的糖和果糖含量的QTL.
- 为了验证QTL区域内的等位基因变异和基因相互作用.
- 发现调节果糖含量的功能遗传变异,提高育种可预测性.
主要方法:
- 使用QTL映射和散装分离分析测序 (BSA-seq) 在果交叉种群中识别QTL.
- 分析了QTL区域内候选基因的等位基因变异及其对基因表达和蛋白质相互作用的影响.
- 基因组学辅助预测 (GAP) 模型被开发并评估了预测的准确性.
主要成果:
- 确定了果果糖和果糖含量的QTL.
- 在MdMYB109促进体中的特定单核酸多态 (SNPs) 通过改变转录因子结合来影响MdMYB109的表达.
- MdMYB109直接激活MdSUT2.2的表达,增加了水果中的糖糖含量. 在MdHXK1中的SNP影响MdbHLH3的酸化,导致MdSUT2.2与MdMYB109的联合激活,进一步增加了糖含量.
- 在MdMYB109和MdHXK1中纳入SNP的基因型数据显著提高了GAP模型对总可溶性固体含量的预测准确度,从0.3758到0.5531.
结论:
- 在MdMYB109和MdHXK1中的功能变异是果糖含量的关键调节者.
- 已识别的SNP及其相互作用为果育种提供了有价值的功能标记.
- 一个更新的GAP模型增强了可预测性,促进了高效的基因组学辅助育种,以提高果的质量.
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