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在两种pinto bean重组杂交线种群中绘制对Sclerotinia白菌的耐药性

Alvaro Soler-Garzón¹, Fernanda Souza Lopes², Jayanta Roy³

¹Irrigated Agriculture Research and Extension Center, Washington State University, Prosser, Washington, USA.

The plant genome

|December 9, 2024

概括

研究人员确定了11个定量特征位点 (QTL) 对于白色菌耐药性在pinto豆. 这些发现提供了缩小的基因组区域和候选基因,通过标记器辅助选择来改善抗病能力.

科学领域:

植物病理学植物病理学
遗传学遗传学是一个
农业科学农业科学

背景情况:

由 *Sclerotinia sclerotiorum* 引起的白色菌对全球普通豆 (*Phaseolus vulgaris* L.) 产量产生重大影响.
由于定量遗传和复杂的遗传因素,培养白菌耐药性是复杂的.

研究的目的:

为了验证和表征皮托干豆中对白色菌的生理性耐药性.
为了识别和缩小与白色菌耐药性相关的定量特征位点 (QTL).

主要方法:

进行了温室吸管测试和现场评估.
使用了经典的定量特征位置 (QTL) 映射和Khufu de novo QTL-seq.
分析了两个Pinto豆重组杂交系种群.

主要成果:

在两个种群中 (P2中的五个,P3中的六个) 确定了11个赋予白菌耐药性的QTL.
发现了新的QTL (P2中的WM1.4,WM11.5;P3中的WM1.5,WM7.7),并验证了主要效应的QTL (WM5.4,WM7.4,WM2.2,WM3.1).
对于避开特征的QTL,如抵制性和晚期成熟度的抵制性和QTL重叠.

结论:

这项研究确定了缩小的基因组间隔和潜在的候选基因白色菌耐药性在pinto豆.
这些研究结果支持标记器辅助的选择策略,以增强平托豆育种计划中的白菌耐药性.

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