来自Ocimum carnosum的精油诱导ROS介导的线粒体功能障碍和HL-60细胞的内在亡
Madhunika Agrawal1, A K Saxena2, Satyam Kumar Agrawal3
1Cellsinvitro Lifesciences Pvt. Ltd., SAS Nagar, Mohali 140308, Punjab, India; Cancer Pharmacology Division, CSIR-Indian Institute of Integrative Medicine, Jammu 180016, India.
概括
来自Ocimum carnosum (EOC) 的精油通过诱导白血病细胞的亡,显示出强烈的抗癌作用. 通过依赖线粒体的途径,EOC降低了细胞活力,这表明了治疗潜力.
科学领域:
- 植物化学 植物化学
- 分子生物学分子生物学
- 癌症研究 癌症研究
背景情况:
- 白血病仍然是一个重大的健康挑战,需要新的治疗药物.
- 自然化合物因其抗癌性质而越来越多地被探索.
- 类物种以其多样化的药用应用而闻名.
研究的目的:
- 研究Ocimum carnosum (EOC) 精油对人类前列细胞白血病HL-60细胞的细胞毒性作用.
- 为了阐明EOC诱导的细胞死亡的机制.
- 评估EOC作为抗癌剂的潜力.
主要方法:
- 细胞活力测试以确定IC50值.
- 形态学分析以识别apoptotic特征.
- 流细胞计,以评估细胞循环分布和DNA碎片化.
- 线粒体膜潜力的评估和细胞染色体c释放分析.
- 西部涂抹测试以评估Bcl-2和酶表达.
主要成果:
- 在24小时内,EOC对HL-60细胞表现出强烈的,剂量和时间依赖的细胞毒性,IC50为0.029μl/ml.
- EOC诱导的亡,其特征是细胞收缩,膜,和核凝结.
- 流细胞测量显示,亚G0细胞数量增加,表明DNA碎片化.
- 通过失去线粒体膜潜力和细胞染色体c释放证实了线粒体通路的参与.
- 经过EOC治疗,导致Bcl-2的耗尽和caspases的激活.
结论:
- EOC对HL-60白血病细胞具有显著的细胞毒性活性.
- 通过内在线粒体途径诱导eoc的亡,涉及cytochromec释放和caspase激活.
- 埃奥克显示出有前途的抗癌性质,为治疗开发提供了进一步的研究.
相关概念视频
01:46Electron Transport Chain: Complex I and II
11.5K
The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
ROS generation is regulated and maintained at moderate levels necessary...
11.5K
01:24Peroxisomes
10.7K
Peroxisomes are specialized organelles present in fungi, plant, and animal cells. It can vary in number, size, morphology, and activity depending on the type of tissue and the nutritional state of the cell. For example, cells with active lipid metabolism, such as adipocytes, neurons, and hepatocytes, have more peroxisomes than other cells in the body. Besides their primary role in breaking down complex organic molecules, peroxisomes can also synthesize specific macromolecules and participate in...
10.7K