通过短时间的光动力学动作脉冲来激活NAD(P) H氧化酶
Xiao Bing Xie1, Yu Shu1, Zong Jie Cui1
1College of Life Sciences, Beijing Normal University, Beijing, China.
概括
尼古丁胺胺二核酸 [NAD(P) H]氧化酶 (NOX) 可以通过光动力学作用来激活. 这种方法允许控制NOX激活,为与活性氧物种相关的疾病提供了新的研究可能性.
科学领域:
- 生物化学 生物化学
- 细胞生物学 细胞生物学
- 生物技术是生物技术.
背景情况:
- 尼古丁胺胺二核酸 [NAD(P) H]氧化酶 (NOX) 产生对生理功能至关重要的活性氧物种.
- 氧化酶的失调与各种疾病有关.
- 现有的NOX抑制剂可用,但控制NOX激活的方法是有限的.
研究的目的:
- 研究用于空间时空控制NOX激活的新方法.
- 通过紫外线A (UVA) 辐射和光动力学作用探索NOX酶的激活.
主要方法:
- 在CHO-K1细胞中单个亚单元NOX5的宫外表达.
- 使用UVA照射 (380nm) 激活,并通过Fura-2光成像监测峰.
- 使用光敏化剂 (硫酸或miniSOG) 的光动力学作用,并评估振荡.
主要成果:
- 紫外线辐射激活了异位体表达的NOX5,诱导了重复的峰.
- 紫外线诱导的振荡被二烯化 (DPI) 抑制,并被单片氧灭器Trolox-C阻.
- 与光敏感剂的光动力学作用还诱导了NOX5和DUOX2表达细胞的持续振荡,这些细胞对DPI敏感和对Trolox-C敏感.
结论:
- 通过短暂的光动力学脉冲来激活NOX酶.
- 这种光动力学激活方法为控制NOX活动提供了一个工具.
- 这些发现表明,针对NOX相关疾病的治疗策略有潜力.
相关概念视频
Photoreceptors and Visual Pathways
5.7K
At the molecular level, visual signals trigger transformations in photopigment molecules, resulting in changes in the photoreceptor cell's membrane potential. The photon's energy level is denoted by its wavelength, with each specific wavelength of visible light associated with a distinct color. The spectral range of visible light, classified as electromagnetic radiation, spans from 380 to 720 nm. Electromagnetic radiation wavelengths exceeding 720 nm fall under the infrared category,...
5.7K
Oxidation of Phenols to Quinones
2.9K
In the presence of oxidizing agents, phenols are oxidized to quinones. Quinones can be easily reduced back to phenols using mild reducing agents. The electron-donating hydroxyl group enhances the reactivity of the aromatic ring, enabling oxidation of the ring even in the absence of an α hydrogen.
o-hydroxy phenols are oxidized to o-quinones and p-hydroxy phenols to p-quinones. Such redox reactions involve the transfer of two electrons and two protons. The reversible redox...
o-hydroxy phenols are oxidized to o-quinones and p-hydroxy phenols to p-quinones. Such redox reactions involve the transfer of two electrons and two protons. The reversible redox...
2.9K
The Photochemical Reaction Center
4.1K
Reaction centers are pigment-protein complexes that initiate energy conversion from photons to chemical entities. Therefore, photochemical reaction center is a more appropriate term that describes these complexes. The Nobel laureates Robert Emerson and William Arnold provided the first experimental evidence of photochemical reaction centers by demonstrating the participation of nearly 2,500 chlorophyll molecules for the release of just one molecule of oxygen. Despite thousands of photosynthetic...
4.1K
The Z-Scheme of Electron Transport in Photosynthesis
9.9K
The light reactions of photosynthesis assume a linear flow of electrons from water to NADP+. During this process, light energy drives the splitting of water molecules to produce oxygen. However, oxidation of water molecules is a thermodynamically unfavorable reaction and requires a strong oxidizing agent. This is accomplished by the first product of light reactions: oxidized P680 (or P680+), the most powerful oxidizing agent known in biology. The oxidized P680 that acquires an electron from the...
9.9K
Radical Autoxidation
2.1K
The oxidation of an organic compound in the presence of air or oxygen is called autoxidation. For example, cumene reacts with oxygen to form hydroperoxide. Autoxidation involves initiation, propagation, and termination steps. Many organic compounds are susceptible to autoxidation—especially ethers in the presence of oxygen, which form hydroperoxides. Even though this reaction is slow, old ether bottles contain small amounts of peroxide, which leads to laboratory explosions during ether...
2.1K
Anoxygenic Photosynthesis
Anoxygenic photosynthesis is a phototrophic process that captures light energy to drive carbon fixation without producing molecular oxygen. Unlike oxygenic photosynthesis, which utilizes water as an electron donor and releases oxygen, anoxygenic phototrophs use alternative electron donors such as hydrogen sulfide (H₂S), elemental sulfur (S⁰), or thiosulfate (S₂O₃²⁻). This process is carried out by diverse groups of bacteria, including purple bacteria, green...


