通过非特异性过氧酶的E-和Z-性初级酒精的不同氧化反应
Jiacheng Li1, Cristina Duran2, Balázs Pogrányi1
1Department of Chemistry, University of York, Heslington, YO10 5DD, York, U.K.
Angewandte Chemie (International ed. in English)
|December 10, 2024
概括
非特异性过氧酶 (UPO) 能有效地将合醇转化为环氧化物和碳素酸. 这种可扩展的生物催化方法实现了高产量和酶选择性,有助于合成复杂分子,如昆虫激素.
科学领域:
- 生物催化剂是一种生物催化剂.
- 酶工程是什么?酶工程是什么?
- 有机合成 有机合成
背景情况:
- 非特异性过氧酶 (UPO) 是催化选择性氧化反应的酶.
- 过氧化是UPO催化转化所需的唯一氧化剂.
- 来自Agrocybe aegerita的UPO的PaDa-I变体表现出独特的催化性能.
研究的目的:
- 使用PaDa-I UPO变体研究Z-和E-性酒精的选择性氧化.
- 评估UPO催化反应的可扩展性和酶选择性.
- 证明UPO在合成有价值的有机中间体中的实用性.
主要方法:
- 使用PaDa-I UPO.的Z-和E-性酒精的酶氧化.
- 准备性规模反应以确定单独的产量.
- 对于环氧化产品的反体过量测定.
- 在不相干的基体的总合成中的应用.
主要成果:
- PaDa-I UPO催化了醇到环氧化物 (来自Z-异构体) 和碳酸/化物 (来自E-异构体) 的补充氧化.
- 准备性规模反应实现了高达80%的孤立产量.
- 氧化过程具有很好的反选择性 (>99% ee).
- 成功地转化了E/Z混合物,并合成了两种不相干的反体.
结论:
- UPOs,特别是PaDa-I变体,是选择性醇氧化过程中的有效生物催化剂.
- 开发的方法是可扩展的,并提供高的enantioselectivity,适合准备合成.
- UPOs显示了氧化化合物和复杂分子的工业生产的巨大潜力.
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