新型的多种病毒被封装在有线状病毒中
Zhenhao Han1,2,3,4, Jingjing Jiang1,2,3,4,5, Wenxing Xu1,2,3,4
1National Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops, Wuhan, China.
Journal of virology
|December 10, 2024
概括
在真菌菌株中发现了三种新的多菌病毒,证实它们是丝状和封闭的. 一个病毒增强了真菌生长和毒性,为病毒多样性提供了洞察力.
科学领域:
- 病毒学 病毒学
- 菌类学 菌类学是指菌类学.
- 分子生物学分子生物学
背景情况:
- 最近建立的病毒家族Polymycoviridae具有不确定的病毒形态,只有一个已知的成员,Colletotrichum camelliae丝状病毒1 (CcFV1),被证实是封闭的.
- 了解多基冠病毒的结构和多样性对于理解病毒进化和宿主相互作用至关重要.
研究的目的:
- 为了识别和表征来自植物病原菌的新型多种病毒病毒.
- 阐明这些新病毒和其他已知的多种病毒的形态和封装.
- 为了研究多菌病毒感染对真菌宿主生物影响.
主要方法:
- 从新型病毒中识别和测序双链RNA (dsRNA) 基因组.
- 传输电子显微镜 (TEM) 用于可视化病毒粒子形态.
- 免疫吸收电子显微镜 (ISEM) 具有免疫黄金标签,以确认外衣蛋白相互作用和粒子结构.
- 评估病毒感染对真菌生长和毒性的生物影响.
主要成果:
- 在植物病原菌中发现了三种新型多种病毒,PcsPmV1,PhcPmV1和PhcPmV2,它们具有5或6种编码关键蛋白质的基因组dSRNA,包括依赖RNA的RNA聚合酶和富含proline-alanine-serine的外衣蛋白.
- TEM和ISEM分析证实,PcsPmV1,PhcPmVs和Botryosphaeria dothideaRNA病毒1 (BdRV1) 被封装在丝状病毒中,这支持了多基共病毒通常形成丝状粒子的假设.
- 观察到PcsPmV1感染可以增强其真菌宿主的生长和毒性,这是首个报告的多菌病毒病毒对其宿主致病性产生积极影响的例子.
结论:
- 多种病毒总是被封装在有线状病毒中,主要由富含proline-alanine-serine的外衣蛋白组成.
- 发现新型多种病毒扩大了Polymycoviridae家族内的已知多样性,并有助于理解病毒粒子进化.
- 发现PcsPmV1增强了真菌的毒性,这突显了多基共病毒在宿主-病原体相互作用中的重要生物学作用.
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