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Updated: Jun 5, 2025

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
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Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae

Published on: September 11, 2022

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Cas12a/crRNA的识别启动了对色度测量无细胞DNA (cfDNA) 分析的自启动中介链延伸

Ming Li1, Ting Zheng1, Jiaqi Zhu1

  • 1Department of Laboratory Medicine, Tianyou Hospital Affiliated to Wuhan University of Science and Technology, No. 9 Tujialing, Dingziqiao Road, Wuchang District, Wuhan, Hubei province, 430064, China. 13247126393@163.com.

The Analyst
|December 10, 2024
PubMed
概括

这项研究引入了一种使用CRISPR-Cas12a技术检测无细胞DNA (cfDNA) 的新快速方法. 该方法为潜在的 point-of-care 癌症诊断提供了高灵敏度和准确性.

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Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection07:16

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Published on: February 9, 2024

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Fluorescence Based Primer Extension Technique to Determine Transcriptional Starting Points and Cleavage Sites of RNases In Vivo10:51

Fluorescence Based Primer Extension Technique to Determine Transcriptional Starting Points and Cleavage Sites of RNases In Vivo

Published on: October 31, 2014

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相关概念视频

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
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