使用基因组学来探索污水系统中万科米耐药性的流行病学
Emilie Egholm Bruun Jensen1, Saria Otani1, Ivan Liachko2
1National Food Institute, Technical University of Denmark, Kgs Lyngby, Ghana.
Microbiology spectrum
|December 10, 2024
概括
像vanHAX这样的抗菌素耐药性基因在哥本哈根仍然存在.
科学领域:
- 环境微生物学环境微生物学
- 抗微生物耐药性 抗微生物耐药性
- 基因组学就是基因组学.
背景情况:
- 20多年来,在Lynetten下水道系统中,葡萄糖耐药性,特别是vanHAX介导的耐药性,一直很普遍.
- 预计抗微生物耐药细菌的长期殖民将导致多种细菌种群的基因共享.
研究的目的:
- 调查糖抗性基因在vanHAX以外的传播.
- 确定长期殖民是否导致各种细菌种群的耐药性.
- 评估近距离结合 (Hi-C) 技术在复杂环境中将抗性基因与特定细菌宿主结合的实用性.
主要方法:
- 来自三个污水处理厂入口的505个数据集的Shotgun元基因组测序.
- 分析831个糖抗性基因的差异性丰度.
- 来自Lynetten下水道系统的12个样本的近距离结合 (Hi-C) 基因组分析.
- 读取到组装的基因组和NCBI数据库的BLAST映射.
- 对已识别的结合体进行等离子体分析.
主要成果:
- 只有vanHAX和vanHBX基因在Lynetten系统中显示出差异的丰度.
- vanHBX仅与属级 (Bacillota) 相关,主要在肠球菌等离子体上发现.
- 与vanHAX相关的读数与Enterococcus faecium,Enterococcus faecalis,Paenibacillus apiarius和Paenibacillus thiaminolyticus有关,大多数读数与Paenibacillus有关.
- 含有vanHAX的Hi-C结合物主要被标注为Enterococcus物种中发现的等离子体.
- 尽管存在了20年,但vanHAX尚未在多种细菌种群中广泛传播,主要存在于Enterococcus物种和Paenibacillus中.
结论:
- 在过去的二十年中,vanHAX基因集群在Lynetten下水道系统的细菌种群中显示出有限的传播.
- Hi-C技术对于将抗菌素耐药性基因与细菌种群联系起来很有用,但在精确的基因组定位方面面临挑战.
- 范哈克斯的主要宿主似乎是Enterococcus物种,在Paenibacillus有着显著的存在,这表明随着时间的推移,有限的水平基因转移到广泛的种群中.
更多相关视频
08:58Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
Published on: March 3, 2023
6.3K
06:56Detection of Horizontal Gene Transfer Mediated by Natural Conjugative Plasmids in E. coli
Published on: March 24, 2023
5.2K
相关概念视频
01:30Development of Antibiotic Resistance
Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...
00:57Antibiotic Selection
52.3K
Overview
52.3K
00:46Genomic DNA in Prokaryotes
43.4K
The genome of most prokaryotic organisms consists of double-stranded DNA organized into one circular chromosome in a region of cytoplasm called the nucleoid. The chromosome is tightly wound, or supercoiled, for efficient storage. Prokaryotes also contain other circular pieces of DNA called plasmids. These plasmids are smaller than the chromosome and often carry genes that confer adaptive functions, such as antibiotic resistance.
Genomic Diversity in Bacteria
Although bacterial genomes are much...
Genomic Diversity in Bacteria
Although bacterial genomes are much...
43.4K
01:28Gene Regulation in Microbial Communities: Quorum Sensing
1
Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
1