完整基因组序列和体外益生菌评估 Bacillus subtilis DC-11 从传统发酵的Idli Batter中分离出来
Deepti N Chaudhari1, Jayesh J Ahire2, Amit A Kulthe1
1MIT School of Food Technology, MIT-ADT University, Pune, 412201, India.
Current microbiology
|December 10, 2024
概括
Bacillus subtilis DC-11,从伊德利中分离出来,在模拟的胃和肠道条件下表现出极好的益生菌潜力,具有很高的活力. 这种安全的益生菌候选者还能产生抗微生物亚提洛辛A,抑制有害细菌.
科学领域:
- 微生物学 微生物学
- 食品科学 食品科学 食品科学
- 基因组学就是基因组学.
背景情况:
- 益生菌提供健康益处,但需要严格的安全性和有效性评估.
- 细菌细菌是一种经过充分研究的物种,具有已知的益生菌性质.
- 传统的发酵食品是潜在有益的微生物的丰富来源.
研究的目的:
- 为了进行Bacillus subtilis DC-11的体外益生菌评估.
- 执行全基因组测序 (WGS) 进行全面的遗传分析.
- 评估这一新型菌株的安全性和功能性质.
主要方法:
- 在体外检测益生菌性质 (胃/肠道活力,自聚,粘附).
- 现型安全性评估 (肠毒素,粘素降解,抗生素敏感性).
- 全基因组测序 (WGS) 用于遗传安全 (等离子体,ARG,毒性因子).
主要成果:
- B. subtilis DC-11表现出高活力 (88.98%的胃,98.60%的肠道) 和良好的粘合性能.
- 该菌株对肠毒素产生,粘素降解和常见抗生素耐药性的检测结果呈阴性.
- WGS证实没有等离子体,ARG和毒性因子;确定了亚细素A的产生.
结论:
- 细菌细菌DC-11是一种安全且有前途的益生菌候选者.
- 它强大的体外益生菌特性和遗传安全概况支持其潜力.
- 亚细素A的产生增加了它的抗菌能力.
相关概念视频
Microbes in Food Production
Microbial fermentation is central to food biotechnology, enhancing flavor, texture, preservation, and stability. Fermentative microorganisms metabolize carbohydrates into organic acids, alcohols, and other metabolites that inhibit spoilage organisms and improve digestibility while contributing distinctive sensory qualities.In baking, amylases naturally present in flour hydrolyze starch into monosaccharides such as glucose, which Saccharomyces cerevisiae ferments anaerobically. Through...
Microbes in the Production of Fermented Foods
Lactic acid bacteria (LAB) and molds are instrumental in fermenting plant-based foods to enhance preservation and ensure year-round availability. These microbial processes convert plant carbohydrates into organic acids and other metabolites that inhibit spoilage organisms and contribute to the sensory qualities of the final product.In sauerkraut production, cabbage goes through a microbial succession that starts with cocci such as Leuconostoc mesenteroides. These microbes begin fermentation by...
Probiotics
Probiotics are live, non-pathogenic microorganisms that confer health benefits by modulating the gut microbiota. The human gastrointestinal tract harbors a complex microbial ecosystem, and the balance of this microbiota is crucial for digestive and systemic health. Among the most extensively studied and utilized probiotics are species formerly classified within the genera Lactobacillus and Bifidobacterium. These organisms not only naturally colonize the human gut but are also consumed through...


