相关实验视频

Updated: Jun 5, 2025

In Vitro and In Vivo Evaluation of Photocontrolled Biologically Active Compounds - Potential Drug Candidates for Cancer Photopharmacology

In Vitro and In Vivo Evaluation of Photocontrolled Biologically Active Compounds - Potential Drug Candidates for Cancer Photopharmacology

Published on: September 29, 2023

胆固醇内外 - - 通过其细胞内前体的负反调节

Magdalena Kotowska¹, Mateusz Wenecki¹, Bartosz Bednarz²

¹Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Rudolfa Weigla 12, 53-114, Wroclaw, Poland.

Applied microbiology and biotechnology

|December 10, 2024

概括

细胞内胆固醇前体 (preCPK) 在Streptomyces coelicolor中抑制胆固醇生物合成. CpkF出口对细胞外CPK生产至关重要,揭示了抗生素监管中的负反循环.

科学领域:

微生物学微生物学
分子生物学分子生物学
生物化学生物化学

背景情况:

菌株Streptomyces coelicolor A3(2) 是抗生素生产的一个模型生物.
二次代谢物生物合成基因集群 (BGCs) 的调节是复杂的.
生物合成产品可以调节调节器的活动.

研究的目的:

研究细胞内胆固醇前体 (preCPK) 在调节CPK BGC中的作用.
描述集群编码的废流CpkF及其在CPK生产中的作用.
确定细胞内或细胞外的CPK化合物是否调节反调节.

主要方法:

利用基于 luciferase 的记者系统来比较野生型, ΔcpkF 和 ΔcpkC 菌株中的促进体活性.
生成的 ΔcpkF 突变缺少 CPK 出口者.
产生了 ΔcpkC 突变,缺乏 CPK 生产.

主要成果:

细胞内预CPK参与了负反循环,抑制了CPK BGC.
CpkF对于细胞外的CPK产生至关重要.
在ΔcpkC (没有CPK) 中,促进剂活性上调,在ΔcpkF (没有细胞外CPK) 中类似于WT,表明细胞内CPK沉默了自身的产生.

结论:

聚基基基因聚基因作为其自身产生的沉声器,可能通过结合一个未确定的调节蛋白.
CpkF对于出口CPK至关重要,使细胞外积累和反调节成为可能.
这项研究阐明了抗生素生物合成中的新型负反机制.

关键词:

这种菌种是Streptomyces coelicoloror.主要的促进者超级家庭.聚基基化物 (Polyketide) 是一种聚基基化物.规则规则规则规则规则规则报告员系统报告员系统二次新陈代谢二次新陈代谢

更多相关视频

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability

Published on: April 22, 2016

Functional Complementation Analysis FCA: A Laboratory Exercise Designed and Implemented to Supplement the Teaching of Biochemical Pathways

Functional Complementation Analysis FCA: A Laboratory Exercise Designed and Implemented to Supplement the Teaching of Biochemical Pathways

Published on: June 24, 2016

相关概念视频

Feedback Inhibition

Feedback Inhibition

Biochemical reactions are occurring constantly in cells, converting starting substances to different products, usually with the help of enzymes that speed the reactions. Without enzymes, it would take far too long for most reactions to occur to be useful to the cell!

Cell Signaling Feedback Loops

Cell Signaling Feedback Loops

Positive and negative feedback loops are crucial for regulating biological signaling systems. These feedback loops are processes that connect output signals to their inputs.
Negative feedback loops
Most signaling systems have negative feedback loops that can perform different functions such as output limiter, and adaptation.
Output limiter
Upon receiving an input signal, the cellular response rapidly increases until a threshold is reached. Beyond this threshold, a negative feedback loop...

Regulated Protein Degradation

Regulated Protein Degradation

It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...

Drugs that Destabilize Microtubules

Drugs that Destabilize Microtubules

Microtubules are dynamic structures and can be regulated by microtubule targeting agents (MTAs). Microtubule destabilizing drugs are a class of MTAs that destabilize and prevent microtubules' polymerization. Both natural and synthetic chemicals can be found under this class of drugs. Vincristine and vinblastine, two vinca alkaloids, and colchicine were among the first to be discovered. These drugs can affect cells in various ways, either by inducing a change in cell morphology, preventing...

Positive Regulator Molecules

Positive Regulator Molecules

To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.

Antimicrobial Proteins

Antimicrobial Proteins

Antimicrobial proteins are important components of the immune system. They aid the body in combating pathogens by either killing them directly or hindering their replication processes. Four main types of antimicrobial substances are interferons, the complement system, iron-binding proteins, and antimicrobial proteins.
Interferons
Interferons (IFNs) are proteins produced by lymphocytes, macrophages, and fibroblasts infected with viruses. While IFNs cannot prevent viruses from entering and...