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Updated: Jun 5, 2025

Scalable 96-well Plate Based iPSC Culture and Production Using a Robotic Liquid Handling System
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Published on: May 14, 2015

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可扩展的基于生物反应器的悬浮方法,从健康的捐赠者衍生的iPSCs产生干细胞衍生的岛屿

Kevin Verhoeff1,2, Nerea Cuesta-Gomez1,2,3, Jasmine Maghera1,4

  • 1Alberta Diabetes Institute, University of Alberta, Edmonton, AB, Canada.

Transplantation
|December 10, 2024
PubMed
概括

使用垂直轮生物反应器实现了诱导多能干细胞 (iPSC) 到小岛细胞的可扩展分化. 这种方法提高了细胞产量并降低了成本,为iPSC小岛疗法的临床应用铺平了道路.

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Embryonic and induced pluripotent stem cells are excellent models for disease research because of their ability to self-renew and differentiate into most cell types. Somatic cells from a patient are isolated and reprogrammed into induced pluripotent stem cells or iPSCs. These iPSCs are later differentiated into the desired cell type, which mirrors the diseased cell of the patient. In this way, disease models have been created for investigating diseases such as Down syndrome, type I diabetes,...
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Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
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