维生素B6和维生素B12的同时封装使用ain/gum arabic纳米载体,以提高稳定性,生物可访问性和口服生物可用性
Vijaykumar Ramesh Karoshi1, Ilaiyaraja Nallamuthu1, Tamatam Anand1
1Nutrition, Biochemistry and Toxicology Division, Defence Food Research Laboratory (DRDO-DFRL), Mysore, India.
Journal of food science
|December 10, 2024
概括
这项研究开发了新型的ain/gum arabic纳米颗粒,以提高维生素B6和B12的稳定性和生物可用性. 纳米配方显著改善了维生素的输送,并降低了大鼠的同类半氨酸水平.
科学领域:
- 食品科学与技术 食品科学与技术
- 材料科学 材料科学 材料科学
- 生物技术是生物技术.
背景情况:
- 维生素B6和维生素B12是必需的微量营养素,其稳定性和生物可用性有限.
- 开发有效的输送系统对于增强营养吸收和治疗疗效至关重要.
研究的目的:
- 制造和优化一个使用ain/gum arabic (GA) 纳米颗粒 (NP) 的联合交付系统,以提高维生素B6 (氧化) 和维生素B12 (蓝) 的稳定性和生物可用性.
- 描述开发的纳米配方的物理化学性质,体外生物可访问性,细胞吸收和体内生物可用性.
主要方法:
- 使用抗溶剂蒸发方法制备了封装素和蓝素的Zeen/gum arabic纳米颗粒 (PC-ZG NPs).
- 使用Box-Behnken设计来统计优化工艺条件.
- 纳米粒子的特征是使用SEM,XRD和TGA. 在大鼠中评估了稳定性,生物可访问性,细胞吸收和体内生物可用性.
主要成果:
- 优化的PC-ZGNP表现出小尺寸 (~170nm),高泽塔潜力 (-31mV) 和高效封装 (>56%) 的两个维生素.
- 该纳米配方表现出良好的热稳定性,90天内的改善物理稳定性,以及增强的生物可访问性 (56-62%) 和细胞吸收.
- 在老鼠中,口服药物显著增加了相对生物可用性 (B6的4.8倍,B12的2.2倍),并降低了血同型氨酸水平.
结论:
- 开发的Teen/gum arabic纳米配方有效地提高了维生素B6和B12的稳定性和生物可用性.
- 纳米封装改善了物理化学特性和营养物质的输送,表明其作为食品和营养药物应用的有效输送系统的潜力.
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