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相关概念视频

Conservation of Momentum: Problem Solving01:30

Conservation of Momentum: Problem Solving

Solving problems using the conservation of momentum requires four basic steps:
Van de Graaff Generator01:15

Van de Graaff Generator

Van de Graaff generators (or Van de Graaffs) are devices used to demonstrate high voltage due to static electricity that can also be used for research. Robert Van de Graaff first built one in 1931 (based on original suggestions by Lord Kelvin) for use in nuclear physics research.
Van de Graaff uses both smooth and pointed surfaces, conductors, and insulators to generate large static charges and, hence, large voltages. A substantial excess charge can be deposited on the sphere because it moves...
Energy Stored in a Capacitor: Problem Solving01:26

Energy Stored in a Capacitor: Problem Solving

In 1749, Benjamin Franklin coined the word battery for a series of capacitors connected to store energy. Capacitors store electric potential energy that can be released over a short time. This property means capacitors have a wide range of applications.
Capacitor-discharge ignition is a type of ignition system commonly found in small engines where the energy released from a capacitor ignites an induction coil that, in turn, fires the spark plug.
To calculate the energy stored in a capacitor of...
Thomson's e/m Experiment01:19

Thomson's e/m Experiment

In a beam of charged particles created by a heated cathode, the particles move at different speeds. However, many applications need a beam with uniform particle speeds. An arrangement known as a velocity selector uses electric and magnetic fields to pick particles with a particular speed from the beam.
A particle with charge q, speed v, and mass m enters an area from the top, where the magnetic and electric fields are perpendicular both to the particle's motion and to one another. The magnetic...
Atomic Emission Spectroscopy: Overview01:20

Atomic Emission Spectroscopy: Overview

Atomic emission spectroscopy (AES) is an analytical technique used to determine the elemental composition of a sample by analyzing the light emitted from excited atoms. In AES, atoms in a sample are excited to higher energy levels by thermal energy from high-temperature sources, such as plasma, arcs, or sparks. When these excited atoms return to lower energy states, they emit light at specific wavelengths characteristic of each element. The resulting atomic emission spectrum, which consists of...
Atomic Emission Spectroscopy: Lab01:29

Atomic Emission Spectroscopy: Lab

AES is a powerful analytical technique, especially effective when used with plasma sources, producing abundant spectra in characteristic emission lines. The Inductively Coupled Plasma (ICP), in particular, yields superior quantitative analytical data due to its high stability, low noise, low background, and minimal interferences under optimal experimental conditions. However, newer air-operated microwave sources are emerging as promising alternatives that could be more cost-effective than...

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相关实验视频

Updated: May 11, 2026

A Practical Guide to Phylogenetics for Nonexperts
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一个过去的爆发:重新审视blastp的E值.

Yang Young Lu1, William Stafford Noble2, Uri Keich3

  • 1Cheriton School of Computer Science, University of Waterloo, Waterloo, ON N2L 3G1, Canada.

Bioinformatics (Oxford, England)
|December 10, 2024
PubMed
概括
此摘要是机器生成的。

本研究评估了由蛋白质BLAST (blastp) 产生的序列对齐的统计意义. 提出了一种替代方法,比传统的E值提供更准确的显著性估计,改善正确对齐的发现.

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科学领域:

  • 生物信息学是一种生物信息学.
  • 计算生物学 计算生物学
  • 基因组研究 基因组研究

背景情况:

  • 基本本地对齐搜索工具 (BLAST) 对于基因组研究和序列相似性搜索至关重要.
  • BLAST的E值为对齐提供了统计学意义,表明类似得分的预期随机对齐.

研究的目的:

  • 从蛋白质BLAST (blastp) 中批判性地评估E值的准确性.
  • 提出和验证一个替代的统计方法来评估对齐的显著性.

主要方法:

  • 对潜在的保守性或自由性的blastp E值的评估.
  • 开发一种基于采样随机对齐的零分布的新型显著性分析.
  • 在广泛的实验中比较拟议方法的性能与blastp E值.

主要成果:

  • Blastp E值可以不一致的保守或自由.
  • 拟议的方法提供了有效的显著性估计,没有膨胀的得分.
  • 这种新方法往往比blastp的E值少保守,并且可以产生更正确的对齐.
  • 该方法支持灵活选择替换矩阵和差距处罚.

结论:

  • 开发的方法为评估序列对齐显著性的blastp E值提供了更可靠的替代方案.
  • 这种方法提高了在基因组研究中识别生物相关对齐的准确性.
  • 参数的灵活性和错误率控制的可能性比标准BLAST分析提供了优势.