相关实验视频

Updated: Jun 5, 2025

Resolving Water, Proteins, and Lipids from In Vivo Confocal Raman Spectra of Stratum Corneum through a Chemometric Approach

Resolving Water, Proteins, and Lipids from In Vivo Confocal Raman Spectra of Stratum Corneum through a Chemometric Approach

Published on: September 26, 2019

使用深度学习和空间分析检测角质层纳米纹理特征:用于皮肤屏障评估的非侵入性工具

Jen-Hung Wang¹, Jorge Pereda¹, Ching-Wen Du^1,2

¹Department of Health Technology, Technical University of Denmark, Kongens Lyngby 2800, Denmark.

|December 10, 2024

概括

这项研究引入了一种使用深度学习的新方法,用于准确地检测皮肤细胞上的圆形纳米尺寸物体 (CNOs). 这种方法通过分析角质细胞纳米纹理来增强阿托皮性皮炎 (AD) 诊断,提高了对传统方法的准确性.

科学领域:

皮肤病学皮肤病学
生物标志物发现发现
纳米技术纳米技术

背景情况:

角质细胞表面纳米纹理是亚托皮性皮肤炎 (AD) 的潜在生物标志物.
目前用于量化纳米质图像中的圆形纳米尺寸物体 (CNOs) 的方法,由于成像限制,容易出现不准确性.
对于AD评估,需要对角层 (SC) 带剥离进行非侵入性分析.

研究的目的:

开发一种精确和强大的方法来计算角质细胞纳米纹理图像中的CNO密度.
通过克服传统计算机视觉技术的局限性,提高识别CNO的准确性.
建立一个新的指数,以推进AD诊断.

主要方法:

通过高速原子力显微镜收集了来自45名AD患者和15名健康对照者的1000多张角细胞纳米纹理图像.
训练有素的深度学习对象检测器在收集的图像中识别CNO.
实现了核心密度估计器,用于空间分析CNO分布,不包括无效区域.

主要成果:

开发的深度学习模型在检测CNOs时实现了91.4%的准确性.
综合方法证明了对纳米成像器件和遮的稳定性.
有效的 Corneocyte 地形指数 (ECTI) 是为了精确计算 CNO 密度而开发的.

结论:

有效的 Corneocyte 地形指数 (ECTI) 提供了一个精确的方法来计算 CNO 密度.
ECTI显示了改善阿托皮性皮肤炎诊断的巨大潜力.
该方法有效地区分了不同的AD严重程度和健康的对照.

关键词:

原子力显微镜 (AFM) 的使用亚托邦性皮肤炎 (AD)角质细胞的表面地形图.深度学习是一种深度学习.核密度估计器 (KDE) 的核心密度估计器对象检测检测对象检测对象检测

更多相关视频

Improving 2D and 3D Skin In Vitro Models Using Macromolecular Crowding

Improving 2D and 3D Skin In Vitro Models Using Macromolecular Crowding

Published on: August 22, 2016

Measuring and Modeling Contractile Drying in Human Stratum Corneum

Measuring and Modeling Contractile Drying in Human Stratum Corneum

Published on: March 1, 2017

相关概念视频

Cells of the Epidermis

Cells of the Epidermis

The epidermis is made of four or five layers of epithelial cells, depending on its location in the body. From deep to superficial, these layers are the stratum basale, stratum spinosum, stratum granulosum, stratum lucidum, and stratum corneum.
The cells in all these layers except the stratum basale are called keratinocytes, a type of cell that manufactures and stores the protein keratin. The keratinocytes in the stratum corneum are dead and regularly slough away, being replaced by cells from...

Renewal of Skin Epidermal Stem Cells

Renewal of Skin Epidermal Stem Cells

The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular...