Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

12.4K
In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or...
12.4K
Atomic Emission Spectroscopy: Lab01:29

Atomic Emission Spectroscopy: Lab

149
AES is a powerful analytical technique, especially effective when used with plasma sources, producing abundant spectra in characteristic emission lines. The Inductively Coupled Plasma (ICP), in particular, yields superior quantitative analytical data due to its high stability, low noise, low background, and minimal interferences under optimal experimental conditions. However, newer air-operated microwave sources are emerging as promising alternatives that could be more cost-effective than...
149

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Glycolytic inhibitor 2-deoxy-d-glucose attenuates SARS-CoV-2 multiplication in host cells and weakens the infective potential of progeny virions.

Life sciences·2022
Same author

Germline Variants of <i>CYBA</i> and <i>TRPM4</i> Predispose to Familial Colorectal Cancer.

Cancers·2022
Same author

Whole-Exome Sequencing Identifies a Novel Germline Variant in <i>PTK7</i> Gene in Familial Colorectal Cancer.

International journal of molecular sciences·2022
Same author

Metastasis and Mortality in Men With Low- and Intermediate-Risk Prostate Cancer on Active Surveillance.

Journal of the National Comprehensive Cancer Network : JNCCN·2022
Same author

Serum interleukin-6 in seropositive rheumatoid arthritis and response to tocilizumab: An observational study.

European journal of rheumatology·2022
Same author

Comparison of Laryngeal Ultrasound and Cuff Leak Test to Predict Post-Extubation Stridor in Total Thyroidectomy.

Turkish journal of anaesthesiology and reanimation·2022

相关实验视频

Updated: Jun 5, 2025

An Electrochemiluminescence-Based Assay for MeCP2 Protein Variants
07:44

An Electrochemiluminescence-Based Assay for MeCP2 Protein Variants

Published on: May 22, 2020

27.1K

基于机器学习的强大的视觉成像工具,用于电化学发光生物传感.

Abhishek Kumar1, Shashwat Goel2, Sanket Goel1

  • 1MEMS, Microfluidics and Nanoelectronics (MMNE) Lab, Birla Institute of Technology and Science (BITS) Pilani, Hyderabad Campus, Hyderabad 500078, India; Department of Electrical and Electronics Engineering, Birla Institute of Technology and Science (BITS) Pilani, Hyderabad Campus, Hyderabad 500078, India.

Computers in biology and medicine
|December 10, 2024
PubMed
概括

一个新的机器学习应用程序自动化视觉电化学发光 (ECL) 信号分析,提高疾病标志物检测的准确性,如过氧化和葡萄糖. 这种进步为医疗点诊断提供了标准化和效率.

关键词:
生物标志物 生物标志物生物感应是一种生物感应.电化学发光 (ECL) 是一种电化学发光.机器学习是机器学习.护理点的护理点.

更多相关视频

ELIME Enzyme Linked Immuno Magnetic Electrochemical Method for Mycotoxin Detection
12:11

ELIME Enzyme Linked Immuno Magnetic Electrochemical Method for Mycotoxin Detection

Published on: October 23, 2009

14.1K
Author Spotlight: Integrating BRET-Based Assays and Rare Mutation Analysis to Decipher RAF Kinase Regulation in Live Cells
06:44

Author Spotlight: Integrating BRET-Based Assays and Rare Mutation Analysis to Decipher RAF Kinase Regulation in Live Cells

Published on: March 1, 2024

958

相关实验视频

Last Updated: Jun 5, 2025

An Electrochemiluminescence-Based Assay for MeCP2 Protein Variants
07:44

An Electrochemiluminescence-Based Assay for MeCP2 Protein Variants

Published on: May 22, 2020

27.1K
ELIME Enzyme Linked Immuno Magnetic Electrochemical Method for Mycotoxin Detection
12:11

ELIME Enzyme Linked Immuno Magnetic Electrochemical Method for Mycotoxin Detection

Published on: October 23, 2009

14.1K
Author Spotlight: Integrating BRET-Based Assays and Rare Mutation Analysis to Decipher RAF Kinase Regulation in Live Cells
06:44

Author Spotlight: Integrating BRET-Based Assays and Rare Mutation Analysis to Decipher RAF Kinase Regulation in Live Cells

Published on: March 1, 2024

958

科学领域:

  • 生物医学工程 生物医学工程
  • 分析化学 分析化学
  • 机器学习应用 机器学习应用

背景情况:

  • 视觉电化学发光 (ECL) 是一种针对疾病标志物的敏感诊断技术,适合于医疗机构设置.
  • 目前的ECL方法由于手动数据分析而缺乏标准化和准确性,这阻碍了实时应用.
  • 现有的ECL系统通常需要复杂的仪器仪表,限制了可访问性.

研究的目的:

  • 开发一个完全自动化的,机器学习辅助的图形用户界面 (GUI) 应用程序,用于ECL信号测量和管理.
  • 提高基于ECL的生物标志物量化的准确性和标准化.
  • 创建一个用户友好的平台,用于将机器学习应用到原始ECL图像数据.

主要方法:

  • 开发一个独立的GUI应用程序,集成ECL信号处理的机器学习算法.
  • 通过检测过氧化 (H2O2) 和葡萄糖来验证应用程序的性能.
  • 在自动化框架内利用开源图像处理原则.

主要成果:

  • 开发的应用程序实现了H2O2的0.024mM和葡萄糖的0.035mM的检测极限.
  • 定量限值为H2O2的0.074 mM和葡萄糖的0.10 mM.
  • 实时实用性和ECL信号测量准确度的提高.

结论:

  • 自动化的GUI应用程序标准化ECL测量,提高准确性和效率.
  • 该平台可以无地将机器学习应用于ECL数据分析,使得没有深度计算专业知识的用户受益.
  • 开发的系统在其他光学检测方法中具有潜在的应用,例如化学发光,色度测量和光测试.