通过m4C DNA甲基化在蓝藻细菌中的四聚醇生物合成的表观遗传控制
Nils Schmidt1, Nils Stappert2, Kaori Nimura-Matsune3
1Institute of Biosciences, Department of Plant Physiology, University of Rostock, D-18059 Rostock, Germany.
概括
基因甲基化调节细菌中的基因表达. 在Synechocystis中,N4-甲基氨酸对于正确的hemJ基因表达至关重要,影响叶绿素水平和细胞色素.
科学领域:
- 微生物学 微生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 分子生物学分子生物学
背景情况:
- 表观遗传DNA的修改调节了真核生物的基因表达.
- 在细菌基因调节中DNA甲基化的作用是不太了解的.
- 在GGCC动机上,Synechocystis 6803利用DNA甲基转移酶M.Ssp6803II在GGCC动机上形成N4-甲基细胞素 (GGm4CC).
研究的目的:
- 调查DNA甲基化在Synechocystis中的调控意义.
- 确定M.Ssp6803II在基因表达和细胞表型中的作用.
- 确定受DNA甲基化变化影响的特定基因和途径.
主要方法:
- 基因删除 (∆sll0729) 删除DNA甲基转移酶.
- 表型分析 (颜色,叶绿素水平).
- 基因抑制剂分析和测序.
- 基因表达的转录和qPCR分析.
- 用工程变体进行促销者分析.
主要成果:
- 删除sll0729导致蓝色的表型与减少的叶绿素.
- 抑制器突变揭示了hemJ促进体中的GGCC到GGTC变化.
- 在∆sll0729突变体中观察到异常的hemJ表达和四氧化中间体积累.
- 对hemJ促进体的N4-甲基细胞素修饰对于野生类型的色素来说至关重要.
结论:
- 细胞因子N4甲基化对于Synechocystis中hemJ基因的适当表达至关重要.
- 基因甲基化作为一个相关的表观遗传标记在蓝藻细菌.
- 这项研究阐明了一种影响光合作用和色素生物合成的新型调节机制.
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