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相关概念视频

Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

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Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
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Rab Cascades01:25

Rab Cascades

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Rab GTPases act in a regulated cascade during membrane fusion, helping the lipid bilayers mix. The Rab family of proteins are active when bound to GTP, and inactive when bound to GDP. Hence, they act as guanine nucleotide-dependent molecular switches. Rab-GTP recognizes and binds to long or short-range tethering proteins to capture the target vesicle. These tethers coordinate with SNAREs on the vesicle and the target membrane to assemble the trans SNARE complex that locks the mixing bilayers.
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Separation of Sister Chromatids02:17

Separation of Sister Chromatids

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At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
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Combinatorial Gene Control02:33

Combinatorial Gene Control

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Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
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Regulated Protein Degradation02:58

Regulated Protein Degradation

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It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
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Anaphase Promoting Complex00:50

Anaphase Promoting Complex

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The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
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相关实验视频

Updated: Jun 5, 2025

Genome-wide Mapping of Protein-DNA Interactions with ChEC-seq in Saccharomyces cerevisiae
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通过补充SCF型E3链酶进行双BACH1调节

Benedikt Goretzki1, Maryam Khoshouei1, Martin Schröder1

  • 1Discovery Sciences, Novartis Biomedical Research, Basel, Switzerland.

Cell
|December 10, 2024
PubMed
概括

这项研究揭示了氧化应激和瘤基因的调节者BACH1蛋白是如何被两种ubiquitin连接酶控制的. 一个开关机制允许FBXO22或FBXL17根据其结构状态结合BACH1,影响癌症和氧化应激障碍.

关键词:
在 BACH1库林环链酶在F-box在FBXL17在FBXO22S-化半氨酸的修饰结合酶开关氧化应激

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Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale
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Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale

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Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale
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科学领域:

  • 生物化学
  • 分子生物学
  • 细胞生物学

背景情况:

  • 广泛复杂,电车轨道和bric-à-brac域 (BTB) 和CNC同源1 (BACH1) 是细胞氧化应激和瘤基因的关键调节器.
  • BACH1由SCFFBXO22和SCFFBXL17泛素酶进行后翻译调节,但氧化应激下的识别机制尚不清楚.

研究的目的:

  • 阐明FBXO22和FBXL17在氧化应激条件下识别BACH1的机制.
  • 了解控制BACH1后翻译调节的联酶切换机制.

主要方法:

  • 对BACH1 BTB二聚体及其与FBXO22的相互作用进行结构分析.
  • 研究癌症相关突变和囊蛋白修饰对BACH1稳定性和酶结合的影响.
  • 描述FBXL17作为单体识别BACH1.

主要成果:

  • FBXO22在BACH1 BTB二元体的域交换β表中识别了一个四元降解子.
  • 癌症突变和囊蛋白修饰会破坏FBXO22降级的稳定性,从而破坏结合.
  • 这些修改暴露了二聚体接口上的明显降解子,使FBXL17能够结合单体BACH1.

结论:

  • 根据BTB域的稳定性,酶开关机制通过FBXO22和FBXL17调节BACH1.
  • 这些发现为氧化应激反应提供了机理性的见解.
  • 这项研究可以为与氧化应激相关的疾病和癌症的治疗策略提供信息.