使用序列激活光RNA探针对活细胞内源性RNA进行成像
Haifeng Zheng1,2, Xiyu Liu1,2, Luhui Liu1,2
1Optogenetics & Synthetic Biology Interdisciplinary Research Center, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Mei Long Road, Shanghai 200237, China.
Nucleic acids research
|December 10, 2024
概括
研究人员开发了一种新的序列激活光RNA (SaFR) 技术,用于成像RNA. 这种多功能工具能够对细胞中的RNA进行强大的可视化,有助于理解RNA功能和分子机制.
科学领域:
- 分子生物学分子生物学
- 生物化学 生物化学
- 细胞生物学 细胞生物学
背景情况:
- RNA分子对于许多细胞过程至关重要,包括基因调节和染色体维护.
- 精确的可视化RNA在其原生细胞环境中对于理解其功能至关重要.
- 现有的RNA成像技术在特异性,稳定性和实时跟踪方面存在局限性.
研究的目的:
- 开发一种新的,特定于序列的方法,用于在活细胞和固定细胞中进行光标记和成像RNA.
- 创建一个多功能工具,克服当前RNA成像技术的局限性.
- 证明新技术在跟踪细胞内的动态RNA过程中的实用性.
主要方法:
- 开发一个序列激活光RNA (SaFR) 系统,利用一个依赖于目标的形状变化.
- 基RNA结构的设计,这些结构在与特定的基RNA序列结合时被激活.
- 在细胞模型中应用SaFR用于成像外源和内源RNA.
- 使用SaFR来监控应力颗粒组装和拆卸的实时动态.
主要成果:
- 该 SaFR 技术展示了高特异性,大动态范围和快速光信号生成.
- 在活细胞和固定细胞系统中成功对外源和内源RNA进行成像.
- 在实时中,SaFR有效地监控了应力颗粒的动态组装和拆卸.
- 该系统表现出强大的性能,使RNA局部化和动态的清晰可视化.
结论:
- SaFR为细胞内内源性RNA的标记和成像提供了一个强大而通用的平台.
- 这种技术有助于研究RNA的定位,功能和分子机制.
- 通过实现精确的RNA可视化,SaFR是推动分子生物学和细胞生物学研究的宝贵工具.
相关概念视频
02:31In-situ Hybridization
9.2K
In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
9.2K
03:21RNA-seq
9.8K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.8K
03:31Labeling DNA Probes
8.1K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.1K
02:07FISH - Fluorescent In-situ Hybridization
19.6K
Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
19.6K