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Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...
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Sulfur is an essential element in biological systems, contributing to synthesizing key biomolecules, including amino acids such as cysteine and methionine, and cofactors such as coenzyme A and biotin. Microorganisms primarily assimilate sulfur as sulfate (SO₄²⁻) from the environment, which must undergo a series of biochemical transformations before it can be incorporated into cellular components. As sulfate is highly oxidized, it must undergo assimilatory sulfate reduction to...

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Using an Extracellular Flux Analyzer to Measure Changes in Glycolysis and Oxidative Phosphorylation during Mouse Sperm Capacitation
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在人类精子电容化过程中,脂分子调节氧化还原信号.

Steven Serafini1,2,3, Cristian O'Flaherty1,2,3,4,5

  • 1Experimental Medicine Division, Department of Medicine, McGill University, Montréal, QC, Canada.

Human reproduction (Oxford, England)
|December 10, 2024
PubMed
概括

斯芬哥辛1-酸盐 (S1P) 对于人类精子变得肥沃至关重要. 它激活特定的受体并促进活性氧物种的产生,这对于精子电容和受精能力至关重要.

关键词:
人类的精子,人类的精子.脂质信号传递 脂质信号传递氧化氧化是什么?有活性氧物种的反应性氧物种.氧化还原信号的信号.

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科学领域:

  • 生殖生物学 生殖生物学
  • 脂质信号传递的方法
  • 精子生理学 精子生理学

背景情况:

  • 包括S1P在内的生物活性脂调节了关键的细胞功能,如膜信号传递,增殖和迁移.
  • 了解脂的作用对于生殖健康和生育研究至关重要.

研究的目的:

  • 阐明脂质,特别是S1P在调解人类精子容量的作用.
  • 为了确定S1P诱导的精子功能所涉及的特定信号通路和分子相互作用.

主要方法:

  • 来自健康捐赠者的人类精子被化,包括或不包括脂 (sphingosine,ceramide) 和抑制剂.
  • 试验包括蛋白质氨酸酸化,体反应,氧化生产和精子活力/运动性分析.
  • 免疫细胞化学被用来定位关键的信号分子.

主要成果:

  • S1P通过激活S1PR1受体来调解人类精子电容,从而导致氧化和超氧化等反应性氧物种 (ROS) 的增加.
  • S1P信号激活PI3K-AKT通路,这对于电容期间的氧化生产至关重要.
  • 斯芬戈辛和胺促进ROS的产生,这对于精子受精能力至关重要.

结论:

  • 脂代谢物对于诱导人类精子电容性至关重要,涉及PI3K/AKT/NOS等途径.
  • 新的见解包括蛋白质激酶R (PKR) 在激活氨酸激酶1 (SphK1) 和S1P信号传递中的作用.
  • 这些发现突出了不育男性的生育治疗和诊断的潜在目标.