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Microgel-Extracellular Matrix Composite Support for the Embedded 3D Printing of Human Neural Constructs

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Published on: May 5, 2023

用3D打印的微结构酸盐支架用于神经组织工程.

Jianfeng Li¹, Benjamin Hietel², Michael G K Brunk¹

¹Max Planck Institute of Microstructure Physics, Weinberg 2, Halle, 06120, Germany; Max Planck-University of Toronto Centre for Neural Science and Technology, Toronto, Canada.

Trends in biotechnology

|December 10, 2024

概括

这项研究引入了微结构酸盐支架用于神经元培养,增强细胞粘附和网络形成,无需添加剂. 这些新的支架显示了神经再生和有机体研究的前景.

关键词:

在3D神经元培养中.通过3D打印打印3D打印.酸是一种酸.神经组织工程神经组织工程有孔的脚手架是多孔的脚手架.氧化物四足动物氧化

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Core/shell Printing Scaffolds For Tissue Engineering Of Tubular Structures

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科学领域:

生物材料科学生物材料科学
组织工程是组织工程.
神经科学是一个神经科学.

背景情况:

酸 (Alg) 是用于脚手架工程和生物油墨的常见生物聚合物.
酸缺乏内在的细胞结合部位,因此需要功能化以进行细胞粘附.
目前用于含有细胞的酸油墨的3D生物打印方法因剪切应力而面临消毒和细胞活力的挑战.

研究的目的:

开发无生物活性添加剂,微结构酸盐 (M-Alg) 支架用于神经元培养.
使用ZnO微粒作为牺牲模板,创建具有相互连接的通道和纹理表面的支架.
为了评估与原始阿尔金酸盐相比,M-Alg支架上的神经元粘附,生长和网络形成.

主要方法:

微结构酸盐支架 (M-Alg) 的制造,使用四足形的ZnO (t-ZnO) 微粒作为模板.
清除t-ZnO颗粒以创建多孔,微结构的支架.
在M-Alg支架和原始阿尔金酸盐 (P-Alg) 支架上培养神经元.
评估细胞粘附,神经细胞增长和神经活动.

主要成果:

与P-Alg支架相比,神经元在M-Alg支架上显著改善了粘附和生长.
在M-Alg支架上观察到大量的神经细胞外生和自发的神经活动.
这些M-Alg支架促进了神经元网络的成熟.

结论:

微结构酸盐支架 (M-Alg) 提供增强的神经元粘附和网络形成,而不需要生物活性添加剂.
使用牺牲性ZnO微粒是一种有效的方法,用于创建多孔,纹理的酸盐支架.
这些无添加剂,神经元亲属的M-Alg支架对神经再生医学和有机体研究具有前景.