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相关概念视频

CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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Homologous Recombination02:31

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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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相关实验视频

Updated: Jun 5, 2025

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
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在使用CRISPR-Cas12a的DNA信息存储中随机消毒

Hongyu Shen1, Zhi Weng1, Haipei Zhao1

  • 1School of Biomedical Engineering, The International Peace Maternity and Child Health Hospital, Zhangjiang Institute for Advanced Study and National Center for Translational Medicine, Shanghai Jiao Tong University, Shanghai 200240, China.

Journal of the American Chemical Society
|December 10, 2024
PubMed
概括

本研究使用CRISPR-Cas12a (RSDISC) 进行DNA数据存储的随机消毒. 这种方法可安全地删除DNA文件,实现高效率并实现安全的数据管理.

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科学领域:

  • 生物技术
  • 基因组学
  • 信息科学

背景情况:

  • DNA数据存储为元数据提供了高密度和稳定性.
  • 目前的DNA存储缺乏安全的数据清除方法.

研究的目的:

  • 开发一种安全有效的DNA信息存储随机消毒方法.
  • 能够选择性地删除文件并提高DNA存储系统中的数据安全性.

主要方法:

  • 使用CRISPR-Cas12a对单链DNA (ssDNA) 的附带分裂.
  • 用于选择性ssDNA降解的受控的原料模板混合动力学.
  • 在使用CRISPR-Cas12a (RSDISC) 的DNA信息存储中应用随机消毒.

主要成果:

  • 在一次回合中达到高达99.9%的消毒效率.
  • 基于混合效率模型,已经证明可以删除高达10^12个文件.
  • 展示了不同GC含量,长度和结构的SSDNA选择性降解.

结论:

  • RSDISC提供了一个强大的随机消毒方法来存储DNA数据.
  • 这种方法支持信息加密,文件分类和内存分配.
  • 建立了DNA存储中安全准确数据处理的基础.