塑化剂对等离子体蛋白结合测量的干扰
Makayla Harrison1,2, Samantha Jordan1, Li Di3,4
1Pfizer Worldwide Research and Development, Groton, CT, USA.
The AAPS journal
|December 10, 2024
概括
来自PVC袋中的可塑剂干扰药物结合测量,影响血蛋白结合 (PPB) 数据. 建议使用玻璃管而不是PVC袋进行血液采集和血存储,以获得准确的PPB结果.
科学领域:
- 药理学 药理学是指药理学的学科.
- 分析化学 分析化学
- 生物化学 生化学
背景情况:
- 精确的血蛋白结合 (PPB) 测量对于药物发现至关重要,特别是对于高度结合的化合物.
- 不断发展的方法旨在提高PPB数据质量,需要高质量的等离子体和敏感的检测方法.
- 从聚乙烯 (PVC) 袋中出的可塑剂可以干扰药物结合测试.
研究的目的:
- 研究来自PVC血储存袋的增塑剂对与人类α1-酸性糖蛋白 (AAG) 和人血清白蛋白 (HSA) 的药物结合的干扰.
- 为了比较血蛋白结合 (PPB) 测量,使用储存在PVC袋中的血与玻璃管中的血.
- 为了确定可靠的PPB数据的最佳收集和存储方法.
主要方法:
- 对几个AAG和HSA结合药物的血分数未结合 (fu,p) 值进行比较.
- 血液和血通过吸尘器收集并使用PVC袋或玻璃管进行储存.
- 用聚烯注射器和聚乙烯管收集的动物血中PPB的评估.
主要成果:
- 与储存在玻璃管中的血相比,储存在PVC袋中的血显示出明显更高的fu,p值,差异高达32倍.
- 对某些与AAG和HSA结合的药物观察到可塑剂干扰.
- 使用聚烯注射器和聚乙烯管收集的动物物种血没有显示干扰.
结论:
- 由于可塑化剂漏,PVC等离子储存袋可能会对PPB测量产生重大干扰.
- 建议在PVC袋上使用吸尘器和玻璃管进行血液采集和血存储,以确保准确的PPB数据.
- 适当的阳性对照 (例如,华法林用于HSA,伊马替尼用于AAG) 对于监测血质量和最大限度地减少塑化剂干扰至关重要.
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