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Pseudomonas aeruginosa 的基因组流行病学序列类型 111

Yasufumi Matsumara¹, Gisele Peirano^2,3, Marleen Kock⁴

¹Kyoto University Graduate School of Medicine, Kyoto, Japan.

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology

|December 10, 2024

概括

Pseudomonas aeruginosa ST111,一种多抗药克隆,在加拿大表现出多样化的进化. 在ST111-C2进化中的关键因素包括血清型切换和获得特定的抗菌素耐药性基因.

科学领域:

微生物学微生物学
基因组学就是基因组学.
流行病学流行病学

背景情况:

Pseudomonas aeruginosa 序列类型111 (ST111) 是一个具有全球意义的多重耐药性 (MDR) 高风险克隆.
有限的数据存在于分子流行病学和进化史的ST111在加拿大.

研究的目的:

描述加拿大的ST111分离物的收集,导致血流感染.
研究加拿大和全球ST111种群之间的基因组关系.
为了阐明ST111类的进化历史.

主要方法:

整个基因组测序 (WGS) 使用长短读取10个加拿大ST111分离物 (2010-2018).
全球ST111集合的遗传学分析 (n=969).
使用BEAST软件调查的类型的进化历史.

主要成果:

ST111包括三个分类 (A,B,C) 和两个分类 (C1,C2),ST111-A作为祖先分类.
叶片B,C1和C2在1700-1800年代出现,ST111-C2在全球占主导地位.
ST111-C2的进化涉及血清型切换 (O4到O12) 和获得Tn21,特定的抗性基因 (gyrA_T83I,parC_S87L) 和blaVIM-2/aacA29.

结论:

详细了解不同ST111类的出现和随着时间的推移而演变.
血清型切换和获得抗微生物耐药性决定因素和移动遗传元素显著塑造了ST111-C2的进化.

关键词:

基因组流行病学基因组流行病学在MDR P. aeruginosa克隆中使用.ST111 一个人的生活.

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