基于光报告器的光纤探测器用于连续检测抗体
Suman Nandy1,2, Binh V Vu1, Vijay M Maranholkar3
1William A. Brookshire Department of Chemical and Biomolecular Engineering, University of Houston, Houston, Texas, USA.
Biotechnology and bioengineering
|December 11, 2024
概括
一个新的光纤生物传感器可以实时在线监测人体免疫球蛋白G (IgG) 度. 这项技术可以在生物处理流体中提供精确的检测,这对于制造和质量控制至关重要.
科学领域:
- 生物技术是生物技术.
- 分析化学 分析化学
- 工艺工程的过程工程.
背景情况:
- 准确测量抗体和抗体融合蛋白度对于生物制药工艺开发和制造至关重要.
- 当前的监控方法可能不适合连续的线内应用,限制实时过程控制.
- 分子特异检测对于复杂细胞培养基中可靠的抗体量化至关重要.
研究的目的:
- 开发和验证人类免疫球蛋白G (IgG) 度的连续在线监测平台.
- 评估传感器在生物处理条件下实时检测IgG的性能.
- 为了证明传感器对蛋白质A列监测和生物反应器位数评估等应用的实用性.
主要方法:
- 使用可更换的传感器尖端开发了一种光纤生物传感器.
- 传感器尖端与蛋白质A的Z域的标记的 pentamer 被共地功能化.
- 该平台测试了与人类IgG的度依赖光增强以及在中国大子卵巢 (CHO) 细胞培养液中的特异性.
主要成果:
- 生物传感器证明了人类IgG的度依赖的光增强在0.01-0.75g/L的范围内.
- 在不同IgG度 (1和0.1g/L) 的多次运行中观察到一致的信号.
- 传感器在复杂的CHO细胞培养液的存在下保持了特异性,并且可以在20秒内检测出蛋白质A列的5%突破.
结论:
- 开发的光纤生物传感器为连续的,在线的,实时的IgG监测提供了一个强大的平台.
- 该技术适用于各种上游和下游生物工艺应用,包括工艺控制和泄漏检测.
- 传感器在没有先前样本准备的情况下运行的能力提高了它在动态生物制造环境中的应用性.
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