长非编码RNA ALEX1 赋予ARF3 的功能阶段状态,以增强大米对细菌病原体的抗性
Meng-Qi Lei1, Rui-Rui He1, Yan-Fei Zhou1
1Guangdong Provincial Key Laboratory of Plant Stress Biology, State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, P.R. China.
Molecular plant
|December 11, 2024
概括
一种新的长非编码RNA,ALEX1,调节大米中的蛋白质凝聚,以增强病原体抵抗力. 亚历克斯1与ARF3结合,促进其功能状态并增强对细菌瘟疫的防御能力.
科学领域:
- 植物分子生物学 植物分子生物学
- 植物与微生物的相互作用
- 遗传学和基因组学 遗传学和基因组学
背景情况:
- 米细菌的破坏严重影响全球作物产量.
- 了解植物防御机制对于可持续农业至关重要.
- 长非编码RNAs (lncRNAs) 正在成为植物免疫的关键调节者.
研究的目的:
- 为了研究大米中病原体诱导的 lncRNA ALEX1 的功能.
- 阐明ALEX1赋予病原体耐药性的分子机制.
- 探索lncRNAs在植物中调节蛋白相分离中的作用.
主要方法:
- 定位研究以确定ALEX1的细胞区.
- 生物化学测定证实ALEX1与Auxin响应因子3 (ARF3) 的直接结合.
- 在ALEX1.1的存在下分析ARF3凝结物的特性和动态.
- 对ARF3目标基因的基因表达分析,包括JAZ13.
- 评估米的病原体耐药性.
主要成果:
- ALEX1定位在核中,并直接结合到ARF3.3的内在无序的中间区域 (MR).
- ALEX1调节了ARF3核凝聚物的形成和动态,促进了分散的二维功能状态.
- 这种ALEX1介导的调节增强了ARF3对JAZ13.3等目标基因的转录抑制.
- 吉斯蒙酸信号通路的调节导致对大米细菌病菌的抗性增强.
结论:
- 亚历克斯1 lncRNA通过调节蛋白质凝聚和复杂组合,在大米免疫力中发挥关键作用.
- ALEX1 直接调节 ARF3 阶段分离和功能,影响下游防御通路.
- 这项研究揭示了一种由IncRNA介导的基因调节的新机制,有助于植物病原体的耐药性.
相关概念视频
02:39lncRNA - Long Non-coding RNAs
8.5K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.5K
01:23Types of RNA
63.2K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
63.2K
02:15Experimental RNAi
6.1K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.1K
01:23RNA Interference
25.9K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
25.9K
02:28Leaky Scanning
5.1K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.1K
01:23Transcriptional Regulation: Riboswitches
1
Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...
1