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Overview Of Cell Separation And Isolation

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

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Updated: Jun 5, 2025

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

单细胞隔离芯片与多色条形码阵列集成,用于组织样本中高通量单细胞外体分析.

Chao Wang¹, Yu Zhang^1,2, Jianbo Wang³

¹Institute of Marine Science and Technology, Shandong University, Qingdao, 266237, China.

Advanced materials (Deerfield Beach, Fla.)

|December 11, 2024

概括

一个新的平台可以对外体进行单细胞分析,揭示癌细胞子组中的关键差异. 这项技术有助于理解外体组异质性及其在瘤进展和转移中的作用.

关键词:

对外群体进行分析.这是一个微流体芯片.用光热驱动的隔离装置一个单细胞的单细胞.

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Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

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Published on: April 7, 2023

相关实验视频

Last Updated: Jun 5, 2025

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

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Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

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科学领域:

生物化学生物化学
分子生物学分子生物学
癌症研究癌症研究

背景情况:

外体是癌症进展的关键生物标志物,影响瘤形成,生长和转移.
目前大量的外体细胞检测方法丢失了母细胞信息,忽略了外体细胞异质性.
分析单细胞外体的功能异质性对于理解癌症至关重要.

研究的目的:

开发一个高通量平台,用于单细胞隔离和多颜色外体体表型分析.
为了量化单细胞分泌的微量外体.
揭示来自不同癌细胞亚群的外体的功能异质性.

主要方法:

用光热驱动的单细胞芯片,以实现高效的单细胞隔离 (在5分钟内达到97%的效率).
乳腺癌外体表型蛋白质的质谱和蛋白质相互作用分析.
来自乳腺癌细胞系和临床组织的数万个单细胞的超高吞吐量分析.

主要成果:

在乳腺癌细胞系中识别关键的外体表型,包括CD44和EGFR共同表达子组.
在复杂的瘤微环境中发现免疫逃避PD-L1高表型外体组子组,特别是在HER2阳性组织中.
在外体体表达特征中显示显著的子组差异.

结论:

开发的平台能够进行强大的单细胞隔离,外体细胞量化和表型分析.
这项技术在癌症研究中促进了对单细胞外体组异质性的理解.
这些发现提供了对外体在瘤进展和免疫逃避中的作用的见解.