相关实验视频

Updated: Jun 5, 2025

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay

Published on: June 9, 2017

在高葡萄糖诱导的近端管状上皮细胞中,DNA损伤诱导的AIM2热

Lu'an Li^1,2, Li Zhang², Yating Cai²

¹School of Medicine, South China University of Technology, Guangzhou, China.

Frontiers in cell and developmental biology

|December 11, 2024

概括

在黑色素瘤2 (AIM2) 缺失的炎症酶激活在糖尿病病中驱动热. 针对AIM2可能为这种情况提供新的治疗策略.

科学领域:

腎臟病學 (nephrology) 是一種醫學.
分子生物学分子生物学
细胞生物学细胞生物学

背景情况:

糖尿病病 (DN) 涉及近端管状上皮细胞死亡.
炎症性细胞死亡的 Pyroptosis 与 DN 有关,但其机制尚未完全理解.
缺席在黑色素瘤2 (AIM2) 感知双链DNA,并触发炎症酶激活,导致热亡.

研究的目的:

为了研究AIM2在糖尿病病中近端管状上皮细胞的灭中的作用.
探索关联高葡萄糖,DNA损伤和AIM2-介导的热的潜在机制.

主要方法:

在DN患者和db/db小鼠的管中评估AIM2表达.
使用高葡萄糖 (HG) 诱导的人类-2 (HK2) 细胞进行体外研究.
使用siRNA进行了AIM2和Gasdermin D (GSDMD) 敲击实验.
研究了N-乙半氨酸 (NAC) 对活性氧物种 (ROS) 和热的作用.

主要成果:

在DN脏和HG治疗的HK2细胞中观察到AIM2表达的升高和烧灭酶通路的激活 (分裂-酶-1,GSDMD).
GSDMD的淘汰减少了HG诱导的HK2细胞死亡.
在HK2细胞中减轻了HG诱导的Pyroptosis.
NAC治疗减轻了HG诱导的DNA损伤,AIM2激活和热.
DN患者活检中的AIM2水平与血清肌素相关,与EGFR相反.

结论:

高葡萄糖诱导的DNA损伤激活了AIM2炎症体,导致DN中的近接管状上皮细胞烧灭.
向AIM2为治疗糖尿病病提供了一个潜在的治疗策略.

关键词:

在AIM2中,AIM2是AIM2.造成的DNA损伤是DNA损伤.糖尿病性脏病糖尿病脏病高葡萄糖的情况.热致灭症是什么?

更多相关视频

Author Spotlight: Combining Proximity Ligand Assay with Gamma-H2AX Staining to Characterize Protein Interactions in DNA Damage Response

Author Spotlight: Combining Proximity Ligand Assay with Gamma-H2AX Staining to Characterize Protein Interactions in DNA Damage Response

Published on: August 2, 2024

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice

Published on: May 2, 2025

相关概念视频

DNA Damage can Stall the Cell Cycle

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...

PI3K/mTOR/AKT Signaling Pathway

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...

Abnormal Proliferation

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...