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长非编码RNA H19作为口腔状细胞癌的预后生物标志物

Kiran Kumar¹, Kaveri Hallikeri¹, Ajay Kumar Oli²

¹Department of Oral and Maxillofacial Pathology and Microbiology, SDM College of Dental Sciences and Hospital, Shri Dharmasthala Manjunatheshwara University, Dharwad, Karnataka, India.

Frontiers in medicine

|December 11, 2024

概括

低H19表达与口腔状细胞癌 (OSCC) 的较差结果有关,特别是在吸烟者中. 这种长非编码RNA (lncRNA) 可能成为OSCC的新预后标志物,可能指导向治疗.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

长非编码RNA (lncRNA) H19与瘤进展有关,但其在口腔状细胞癌 (OSCC) 中的作用尚未完全理解.
H19表现出上下文依赖的功能,作为瘤抑制剂和促进剂,受烟草使用等因素的影响.

研究的目的:

为了研究H19在OSCC组织中的表达.
为了将H19表达与临床病理特征,烟草习惯和患者生存结果相关联.
评估H19作为潜在的OSCC复发和存活的独立预后生物标志物.

主要方法:

量化逆转录PCR (qRT-PCR) 用于测量96个OSCC组织和30个相邻的正常粘膜 (NM) 组织中的H19表达.
使用了统计分析,包括t测试,ANOVA,卡普兰-梅尔曲线,日志等级测试和多变量考克斯回归.
使用TCGA OSCC数据库进行比较表达式分析.

主要成果:

与NM相比,H19在OSCC组织和TCGA数据库中表达明显低.
下部H19表达与烟草吸烟习惯有显著的相关性.
低H19表达,以及淋巴结转移,独立预测OSCC患者的整体存活率较差.
较高的年龄,较先进的TNM阶段和较低的H19表达是局部区域复发的独立预测因素.

结论:

H19 作为口腔状细胞癌 (OSCC) 的新预后标志物.
这些发现表明H19可能为OSCC治疗提供治疗点.
烟草使用似乎影响OSCC中的H19表达.

关键词:

H19表达式的表达方式地方区域的复发情况.长非编码RNA是什么意思口腔状细胞癌的发生.总体存活率总体存活率反转录酶PCR是一种反转录酶PCR.

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