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固定时间对人类甲固定嵌大脑组织信使RNA检测能力的影响

Charlene-Annett Hurler1, Sabine Liebscher2,3,4,5, Thomas Arzberger6,7

  • 1Institute for Stroke and Dementia Research, LMU University Hospital, Ludwig-Maximilians-University Munich, 81377 Munich, Germany.

Brain communications
|December 11, 2024
PubMed
概括

固定时间对死后人类脑组织中mRNA检测有重大影响. 标准化固定协议对于神经病理学研究中可靠的mRNA丰度研究至关重要.

关键词:
RNA范围的范围RNA范围的范围人类FFPE组织组织.它们是mRNARNA.死后脑组织的脑组织.转录学方法的方法.

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Fixation and Sectioning01:03

Fixation and Sectioning

Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...