相关实验视频

Updated: Jun 5, 2025

A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli

A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli

Published on: December 9, 2017

在细菌中多重氨酸甲基化的一个新目标

Shori Inoue¹, Shogo Yoshimoto¹, Katsutoshi Hori¹

¹Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University, Nagoya, Aichi, Japan.

Journal of bacteriology

|December 11, 2024

概括

这项研究确定了KmtA是Acinetobacter sp.中的新型外膜蛋白 lysine甲基转移酶 (OM PKMT). 托尔5,负责甲基化三元体自传递体粘附素 (TAA) AtaA,影响细菌的粘附和生长.

科学领域:

微生物学微生物学
后翻译修改后翻译修改
细菌生理学细菌生理学

背景情况:

氨酸甲基化是真核生物中关键的翻译后修饰,调节各种生物过程.
它在 prokaryotes 中的作用,特别是在外膜蛋白中,是不太了解的,只有有限的研究专注于病原体.
三聚体自转载体粘附体 (TAA) 是重要的表面蛋白质,参与细菌相互作用.

研究的目的:

为了研究环境细菌Acinetobacter sp.中的外膜蛋白的氨酸甲基化. 输出量 5. 输出量 5. 输出量
为了确定负责TAA的氨酸甲基化的特定酶,AtaA.
探索这种甲基化在格拉姆阴性细菌中的功能和进化影响.

主要方法:

无标签的液体染色体质谱法 (LC-MS) 用于分析Acinetobacter sp.的细胞表面蛋白质. 输出量 5. 输出量 5. 输出量
为了评估已识别的甲基转移酶的作用,生成了一个缺乏KmtA的突变体.
进行了生物信息和集群分析,以调查OM PKMT基因的分布和演变.

主要成果:

在TAA,AtaA上发现多个氨酸残留物在Acinetobacter sp.中被甲基化. 输出量 5. 输出量 5. 输出量
外膜蛋白质氨酸甲基转移酶 (OM PKMT) KmtA被确定为负责AtaA甲基化的酶.
缺乏KmtA导致细胞表面AtaA增加,增强细菌粘附,增长速度减缓.
类似KmtA的OM PKMT基因在グラム阴性细菌中普遍存在,形成了一个与Rickettsia类型分开的独特集群.

结论:

KmtA是一种新型,高特异性的OM PKMT,在Acinetobacter sp.中甲基化TAA AtaA. 输出量 5. 输出量 5. 输出量
ATAA的氨酸甲基化影响细菌细胞表面特性,粘附和生长.
类似KmtA的酶的广泛分布表明,氨酸甲基化在细菌生理学中的作用比以前被认为的更广泛.

关键词:

细菌粘附是一种细菌粘附.后翻译修改后的修改.蛋白质溶氨酸甲基化蛋白质溶氨酸甲基化三重体的自转载体附着物.

更多相关视频

Site Specific Lysine Acetylation of Histones for Nucleosome Reconstitution using Genetic Code Expansion in Escherichia coli

Site Specific Lysine Acetylation of Histones for Nucleosome Reconstitution using Genetic Code Expansion in Escherichia coli

Published on: December 26, 2020

Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays

Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays

Published on: November 29, 2014

相关概念视频

Types of RNA

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...

Prokaryotic Transcriptional Activators and Repressors

Prokaryotic Transcriptional Activators and Repressors

The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...

Histone Modification

Histone Modification

The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...

Transcription Attenuation in Prokaryotes

Transcription Attenuation in Prokaryotes

Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure. Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...

Epigenetic Regulation

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...

Cytoskeletal Proteins in Bacteria

Cytoskeletal Proteins in Bacteria

Bacterial cells were initially considered simple, randomly organized structures lacking a cytoskeleton. However, the discovery of cytoskeleton homologs in bacteria led to the change of this opinion. Bacterial cytoskeletal filaments regulate the cell shape, cell polarity, cell division, and partitioning of plasmids during cell division. It was later discovered that bacterial cytoskeletal proteins, mainly actin and tubulin homologs, are diverse compared to their eukaryotic counterparts. On the...