转录因子VvMYB44-1在高温下降低葡萄藤安托西亚尼生物合成的作用
Xiangpeng Leng1,2, Chen Li1,2, Peipei Wang1
1Institute of Grape Science and Engineering, College of Horticulture, Qingdao Agricultural University, Qingdao 266109, China.
高温会通过激活VvMYB44-1来降低葡萄的颜色,VvMYB44-1是一种抑制安东氨酸合成的抑制剂. 该基因抑制关键的生物合成酶,并与其他蛋白质相互作用,影响葡萄的质量.
科学领域:
- 植物分子生物学 植物分子生物学
- 植物生理学 植物生理学
- 农业科学 农业科学
背景情况:
- 高温会对许多植物中安东的积累产生负面影响.
- 葡萄 (葡萄) 中热感应的安东素减少的特定分子机制尚未完全理解.
研究的目的:
- 鉴定和描述VvMYB44-1在葡萄中高温诱导的安东素减少中的作用.
- 阐明涉及VvMYB44-1在氨酸生物合成中的调节机制.
主要方法:
- 在高温条件下对VvMYB44-1的基因表达分析.
- 在葡萄和烟草 (Nicotiana tabacum) 中进行过度表达研究,以评估VvMYB44-1的功能.
- 对VvMYB44-1与MYB-bHLH-WD40 (MBW) 复合组件和下游基因 (VvDFR,VvUFGT) 的相互作用进行分析.
- 研究VvMYB44-1对细胞因素 (CK) 代谢的影响 (VvLOG8,VvCKX4).
- 在VvMYB44-1中对乙烯响应元素结合因子相关的两抑制 (EAR) 模式的功能分析.
主要成果:
- 葡萄中的高温显著诱导了VvMYB44-1基因表达.
- 过度表达VvMYB44-1抑制了安东的积累和VvDFR和VvUFGT的转录.
- VvMYB44-1通过破坏MBW激活复合体和调节细胞因素水平来抑制安托西亚宁.
- VvMYB44-1的EAR动机对于其抑制氨酸生物合成的功能至关重要.
结论:
- 在葡萄中,VvMYB44-1 作为一种关键的转录抑制剂,对葡萄中高温诱导的抗素减少起作用.
- 这项研究揭示了一个复杂的调节网络,涉及VvMYB44-1,MBW复合体和细胞因素信号,以应对热应激.
- 了解VvMYB44-1的作用为改善恶劣温度条件下的葡萄质量提供了洞察力.
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