相关实验视频

Updated: Jun 5, 2025

Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing

Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing

Published on: October 16, 2018

抗逆转录病毒治疗中断后,艾滋病病毒和免疫风险因素的反弹

S Gianella¹, T Yu², R Wang^2,3

¹Division of Infectious Diseases and Global Public Health University of California San Diego, CA, USA.

The Journal of infectious diseases

|December 11, 2024

概括

早期启动抗逆转录病毒疗法 (ART) 是防止治疗中断后HIV复发的关键. 病毒学,免疫学和人口学因素影响病毒抑制的成功.

科学领域:

病毒学病毒学
免疫学免疫学免疫学
临床医学临床医学

背景情况:

在治疗中断后识别人类免疫缺陷病毒 (HIV) 反弹风险因素对于开发有效的缓解策略至关重要.
了解这些因素有助于设计旨在实现持续病毒抑制的干预措施.

研究的目的:

为了确定抗逆转录病毒治疗 (ART) 中断后HIV复发的风险因素.
探索病毒学,免疫学和人口因素与病毒反弹时间的关联.

主要方法:

在ART中断之前,从感染艾滋病毒的参与者的外周血液单核细胞分析.
测量细胞关联的HIVRNA,总HIVDNA和前病毒多样性 (env基因).
通过流细胞测量进行免疫表型化,并应用Cox比例危险模型来识别风险因素.

主要成果:

晚期ART启动,更高的ART前HIVRNA,增加的HIV总DNA和高细胞HIV转录与更高的反弹风险有关.
较高的前病毒多样性增加了男性和ZPHI队列的反弹风险.
较低的CD4+T细胞数量,效应细胞/终端分化的T细胞增加,以及更多的激活/耗尽的T细胞与更高的反弹风险有关,特别是在早期接受治疗的参与者中.

结论:

早期的ART启动对于持续的病毒抑制至关重要.
考虑到病毒学,免疫学和人口因素的定制干预措施对于控制艾滋病毒至关重要.
总HIVDNA和终端分化的CD8+T细胞成为反弹风险的重要预测因素.

关键词:

在ART中断中断ART中断艾滋病病毒艾滋病病毒艾滋病病毒预测者预测者预测者

更多相关视频

Humanized NOD/SCID/IL2rγnull (hu-NSG) Mouse Model for HIV Replication and Latency Studies

Humanized NOD/SCID/IL2rγ^null (hu-NSG) Mouse Model for HIV Replication and Latency Studies

Published on: January 7, 2019

Oral Combinational Antiretroviral Treatment in HIV-1 Infected Humanized Mice

Oral Combinational Antiretroviral Treatment in HIV-1 Infected Humanized Mice

Published on: October 6, 2022

相关概念视频

Retrovirus Life Cycles

Retrovirus Life Cycles

Retroviruses have a single-stranded RNA genome that undergoes a special form of replication. Once the retrovirus has entered the host cell, an enzyme called reverse transcriptase synthesizes double-stranded DNA from the retroviral RNA genome. This DNA copy of the genome is then integrated into the host’s genome inside the nucleus via an enzyme called integrase. Consequently, the retroviral genome is transcribed into RNA whenever the host’s genome is transcribed, allowing the...

Pulmonary Tuberculosis I

Pulmonary Tuberculosis I

Tuberculosis, often called TB, is a contagious illness primarily caused by Mycobacterium tuberculosis. It mainly affects the lung parenchyma but can also impact other body parts.
Causative Organism
The primary infectious agent causing tuberculosis is Mycobacterium tuberculosis, a slow-growing, acid-fast, aerobic rod that exhibits sensitivity to heat and ultraviolet light. Instances of Mycobacterium bovis and Mycobacterium avium contributing to the development of TB infection are rare.
Mode of...

Viral Mutations

Viral Mutations

A mutation is a change in the sequence of bases of DNA or RNA in a genome. Some mutations occur during replication of the genome due to errors made by the polymerase enzymes that replicate DNA or RNA. Unlike DNA polymerase, RNA polymerase is prone to errors because it is not capable of “proofreading” its work. Viruses with RNA-based genomes, like HIV, therefore accrue mutations faster than viruses with DNA-based genomes. Because mutation and recombination provide the raw material...

Sexually Transmitted Infections

Sexually Transmitted Infections

Sexually transmitted infections (STIs) are diseases transmitted primarily through unsafe sexual interactions. Bacteria, viruses, or parasites cause them and can result in severe health complications if untreated.ChlamydiaThe bacterium Chlamydia trachomatis is responsible for the disease Chlamydia, the most common STI in the United States. This peculiar pathogen requires human cells to reproduce, residing intracellularly. The initial infection often goes unnoticed because it typically does not...

Immune Response Against Viral Pathogens

Immune Response Against Viral Pathogens

The immune system's response to viral infections is a complex and coordinated process involving natural killer (NK) cells, T cell-mediated responses, and antibody-mediated responses.
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...

Immunodeficiency Diseases

Immunodeficiency Diseases

Immunodeficiency disorders are conditions in which the immune system's ability to fight infectious disease and cancer is compromised or entirely absent. The immune system comprises a complex network of cells, tissues, and organs that work together to protect the body from potentially harmful invaders. When this system is deficient or not functioning properly, it leaves the body susceptible to infections, diseases, or other complications.
There are three main causes of immunodeficiency...