TiO2纳米颗粒对毛囊的影响取决于纳米颗粒内部化速率
Ramsés Santacruz-Márquez1,2, Luz Del Carmen Sánchez Peña1, Jodi A Flaws2
1Department of Toxicology, Center for Research and Advanced Studies (Cinvestav), Av. Instituto Politécnico Nacional 2508, Ciudad de México 07360, México.
概括
二氧化纳米颗粒 (TiO2 NPs) 可以被卵巢毛囊内化,可能会损害生殖功能. 这项研究研究了TiO2 NP的毒性及其对激素合成和氧化应激的影响.
科学领域:
- 环境科学 环境科学
- 毒理学 毒理学 毒理学
- 生殖生物学 生殖生物学
背景情况:
- 二氧化纳米颗粒 (TiO2NP) 在消费品中广泛使用,导致环境释放.
- 对TiO2 NP的毒性存在担忧,特别是对生殖健康和卵巢功能.
- 关于TiO2 NP对卵巢毛囊及其功能的影响的数据有限.
研究的目的:
- 为了研究TiO2 NP暴露对性激素合成,氧化应激和抗氧化剂响应在卵巢腹膜毛囊中的作用.
- 描述TiO2 NP在毛囊内部化及其与观察到的毒理学影响的相关性.
主要方法:
- 卵巢毛囊暴露于不同度的TiO2NP (5,25,50μg/ml) 或载体控制器96小时.
- 随着时间的推移,TiO2NP的内部化得到了评估.
- 分析了基因表达 (Bax/Bcl2比率,类固醇基因) 和激素水平 (孕激素,).
主要成果:
- 在 antral 毛囊中观察到TiO2 NP 内化,最高率为25μg/ml,与增加的Bax/Bcl2 mRNA比率相关.
- 50微克/毫升度显示了改变的类固醇基因表达和增加的孕激素和水平.
- 在最低度 (5μg/ml) 时没有观察到显著的效应或内化.
结论:
- TiO2 NP 内化是卵巢毛囊功能受损的第一步.
- TiO2 NPs可以破坏性激素合成,并诱导卵巢毛囊中的氧化应激.
- 需要进一步的研究来了解TiO2 NP暴露对生殖健康的全部影响.
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