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Updated: Jul 20, 2026

A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1
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A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1

Published on: March 18, 2017

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使用G4RP.v2进行细胞RNAG-四重复形状分析的协议.

Jérémie Mitteaux1, David Monchaud1

  • 1Institut de Chimie Moléculaire de l'Université de Bourgogne (ICMUB), CNRS UMR 6302, 9 Avenue Alain Savary, 21078 Dijon, France.

STAR protocols
|December 11, 2024
PubMed
概括

研究人员改进了一种协议,以从人类癌细胞中分离RNA中的G四复合体 (G4s). 这种方法有助于理解这些重要的核酸结构的生物学作用.

关键词:
生物物理学的生物物理.化学 化学 化学遗传学 是一个遗传学.分子生物学分子生物学

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相关概念视频

Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...