多组学揭示了一条被忽视的H2S生产途径,该途径是由来自堆肥的细菌生物发生引起的
Liu Yang1, Yue Sun1, Jingxiao Zhang1
1College of Life Science, Northeast Agricultural University, Harbin 150030, China.
Journal of hazardous materials
|December 11, 2024
概括
DL-propargylglycine (PAG) 通过抑制硫酸盐还原和生物发生路径,显著降低了堆肥中的硫化 (H2S) 排放. 这一发现为控制H2S污染提供了新的策略.
科学领域:
- 环境科学 环境科学
- 微生物学 微生物学
- 生物技术是生物技术.
背景情况:
- 堆肥产生的硫化 (H2S) 排放造成了严重的空气污染和健康风险,主要归因于硫酸盐的减少.
- 通过囊分裂的细菌H2S生物发生是一种已知的应激反应,但其在波动条件下的堆肥排放中的作用尚不清楚.
研究的目的:
- 调查H2S生物生成途径对堆肥排放的贡献.
- 为了评估DL-propargylglycine (PAG) 的有效性,一种囊酶抑制剂,在不同的空气化速率下,在堆肥过程中减轻H2S的产生.
主要方法:
- 在高和低的通风率下添加DL-propargylglycine (PAG) 的堆肥实验.
- 减少H2S排放的分析,细菌群体的多样性,元基因组学和元蛋白组学.
- 微生物纯培养实验验验证PAG的有效性.
主要成果:
- PAG显著抑制了H2S排放的45.52% (高通风) 和19.74% (低通风).
- PAG降低了核心H2S产生细菌的多样性,降低了硫酸盐还原基因,降低了氨酸酶表达的调节,并提高了酶表达的调节.
- 纯培养实验证实了PAG降低H2S生产的能力.
结论:
- 硫酸盐还原和生物发生路径都会在堆肥过程中促进H2S的产生.
- 通过针对这两种途径,PAG有效地抑制H2S排放,提供了一种新的污染控制策略.
- 这项研究突出了被忽视的H2S生物发生途径,为堆肥操作中的环境管理提供了关键的见解.
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