相关实验视频

Updated: Jun 5, 2025

Author Spotlight: Tracing the Ferroptotic Signatures and Cell Death Dynamics in Medulloblastoma for Advanced Therapeutics

Author Spotlight: Tracing the Ferroptotic Signatures and Cell Death Dynamics in Medulloblastoma for Advanced Therapeutics

Published on: March 15, 2024

一个Redox触发的聚合物纳米粒子,用于破坏Redox恒温和增强铁灭症

Yifei Li¹, Shangcong Han¹, Yi Zhao²

¹Department of Pharmaceutics, School of Pharmacy, Qingdao University, Qingdao, 266073, China.

Small (Weinheim an der Bergstrasse, Germany)

|December 12, 2024

概括

这项研究开发了新的纳米颗粒,可以耗尽谷 (GSH) 和增强光动力疗法 (PDT),通过向氧化还原失衡,有效地诱导癌细胞中的铁亡.

科学领域:

生物医学工程生物医学工程
纳米技术纳米技术
癌症研究癌症研究

背景情况:

癌细胞利用强大的氧化还原系统生存,特别是在低氧环境中,抵抗治疗的氧化损伤.
铁灭,一种受调节的细胞死亡形式,可以破坏癌细胞的氧化还原稳定.
准氧化还原失衡是癌症治疗的一个有希望的策略.

研究的目的:

构建对谷氨 (GSH) 敏感的纳米颗粒,旨在诱导癌细胞中的铁亡.
通过持续的GSH耗尽来增强光动力疗法 (PDT) 的疗效.
开发针对深度缺氧瘤细胞的向治疗方法.

主要方法:

通过二硫化键将碳酸无水化酶IX抑制剂 (与Fe复合的protoporphyrin IX) 和epirubicin (EPI) 移植到氨酸 (HA) 上,形成HSPFE.
在HSPFE上加载一个xCT抑制剂 (SAS),以创建SAS@HSPFE纳米粒子用于主动准.
利用瘤细胞中的高GSH度来爆炸性释放EPI,Por-Fe复合物和SAS.
调查GSH耗尽,活性氧物种 (ROS) 生成,芬顿反应和PDT氧气生产的机制.

主要成果:

SAS@HSPFE纳米粒子积极准深度低氧瘤细胞,并释放它们的有效载荷,以应对高度的GSH.
SAS抑制了GSH生物合成,而Por和Fe促进了氧化应激和ROS生成,推动了PDT.
治疗调节了参与铁亡的关键蛋白质,降低了抗氧化防御的调节 (GPX4,SLC7A11) 和上调了亲氧化剂标记物 (ACSL4,TFRC).
在体内研究表明,SAS@HSPFE纳米粒子具有高效的抗瘤作用.

结论:

开发的SAS@HSPFE纳米粒子通过通过GSH耗尽和增强PDT来破坏氧化还原平衡,有效地诱导铁亡.
这种新型纳米粒子系统通过向瘤细胞中的氧化还原失衡,显示出癌症治疗的巨大潜力.
该战略为开发具有更高效率的向癌症治疗提供了新的途径.

关键词:

对于GSH敏感的药物输送是药物输送的过程.铁性化 (ferroptosis) 是一种氧化还原稳态 (redox homeostasis) 是一种反氧化稳态.

更多相关视频

Synthesis of Stimuli-responsive Nanogels using Aqueous One-step Crosslinking and Co-nanopolymerization

Synthesis of Stimuli-responsive Nanogels using Aqueous One-step Crosslinking and Co-nanopolymerization

Published on: January 24, 2025

Polyethyleneimine-coated Iron Oxide Nanoparticles as a Vehicle for the Delivery of Small Interfering RNA to Macrophages In Vitro and In Vivo

Polyethyleneimine-coated Iron Oxide Nanoparticles as a Vehicle for the Delivery of Small Interfering RNA to Macrophages In Vitro and In Vivo

Published on: February 5, 2019

相关概念视频

Necrosis

Necrosis

Necrosis is considered as an “accidental” or unexpected form of cell death that ends in cell lysis. The first noticeable mention of “necrosis” was in 1859 when Rudolf Virchow used this term to describe advanced tissue breakdown in his compilation titled “Cell Pathology”.
Morphological Manifestations of Necrosis
Necrotic cells show different types of morphological appearance depending on the type of tissue and infection. In coagulative necrosis, cells become...

Redox Reactions

Redox Reactions

Redox reactions are vital biochemical processes that underpin energy metabolism in cells. These reactions involve the transfer of electrons between molecules, occurring in tandem as oxidation and reduction. Oxidation refers to the loss of electrons, while reduction denotes their gain. This coupling ensures the seamless flow of electrons through metabolic pathways. For example, in bacterial metabolism, glucose undergoes oxidation to carbon dioxide, while oxygen is simultaneously reduced to...

Oxidation of Phenols to Quinones

Oxidation of Phenols to Quinones

In the presence of oxidizing agents, phenols are oxidized to quinones. Quinones can be easily reduced back to phenols using mild reducing agents. The electron-donating hydroxyl group enhances the reactivity of the aromatic ring, enabling oxidation of the ring even in the absence of an α hydrogen.
o-hydroxy phenols are oxidized to o-quinones and p-hydroxy phenols to p-quinones. Such redox reactions involve the transfer of two electrons and two protons. The reversible redox...