开发先进的 Oral-on-a-Chip:复制复杂的人口微环境
Soo-Rim Kim1,2, Eun-Kyung Min1,2, Choon-Mi Lee1,2
1Department of Health Sciences and Technology, GAIHST, Gachon University, Incheon, 21999, Republic of Korea.
International journal of biological sciences
|December 12, 2024
概括
一个新的3D口腔芯片模型揭示了桃体干细胞对毒素敏感. 在药物开发中,SERPINB2是检测口服毒性的关键生物标志物.
科学领域:
- 口腔生物学 口腔生物学
- 生物材料科学是生物材料的科学.
- 毒理学 毒理学 毒理学
背景情况:
- 口腔健康依赖于多细胞架构中的复杂细胞相互作用.
- 现有的二维和三维体外模型无法充分复制口腔微环境的复杂性.
- 需要先进的模型来准确评估口腔组织反应和毒性.
研究的目的:
- 开发一个先进的3D口腔芯片系统,模仿口腔微环境.
- 为了研究口腔细胞类型对毒性暴露的不同敏感性.
- 确定生物标志物和开发敏感的检测方法,以检测口腔毒性.
主要方法:
- 使用天然聚合物 (原体,氨酸) 和血液因子构建了一个3D口腔芯片系统.
- 结合了多种口腔细胞类型:牙角质细胞,桃体居住干细胞,牙周带纤维细胞和血管内皮细胞.
- 使用基于生物标志物SERPINB2的光连接检测进行毒性评估.
主要成果:
- 桃体内干细胞对毒性暴露的敏感性比纤维细胞和内皮细胞更高.
- SERPINB2被确定为一个关键的生物标志物,在暴露于毒素时,在桃体内干细胞中显著上调.
- 开发的系统允许对口服中毒性的敏感和定量评估.
结论:
- 这种3D口腔芯片系统有效地模拟了口腔微环境和细胞相互作用.
- 塞尔宾B2作为可靠的生物标志物用于检测口腔毒性,特别是在干细胞中.
- 这个综合系统为评估候选药物的口服毒性提供了一个有价值的平台.
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