相关实验视频

Updated: May 5, 2026

Development of a More Sensitive and Specific Chromogenic Agar Medium for the Detection of Vibrio parahaemolyticus and Other Vibrio Species

Development of a More Sensitive and Specific Chromogenic Agar Medium for the Detection of Vibrio parahaemolyticus and Other Vibrio Species

Published on: November 8, 2016

对Vibrio parahaemolyticus的流行病学截止值是根据在35°C和28°C时生成的最小抑制度数据计算的

Peter Smith¹, Andrew Joseph^2,3, Craig Baker-Austin^2,3

¹School of Natural Science, University of Galway, Galway H91 TK33, Ireland.

Diseases of aquatic organisms

|December 12, 2024

概括

这项研究确定了Vibrio parahaemolyticus抗菌素耐药性的流行病学截止值,这对于准确的敏感性测试至关重要. 化温度影响了一些最小抑制度 (MIC) 结果,突出了对标准化方案的需求.

科学领域:

微生物学微生物学
抗微生物耐药性抗微生物耐药性
兽医公共卫生兽医公共卫生

背景情况:

维布里奥帕拉海莫利蒂库斯 (Vibrio parahaemolyticus) 是一种重要的食物传播病原体.
准确的抗微生物敏感性测试 (AST) 对临床和兽医治疗决策至关重要.
流行病学切线值 (ECOFF) 对于解释AST数据至关重要.

研究的目的:

为10种抗微生物药物针对Vibrio parahaemolyticus.生成数据,以建立针对10种抗微生物药物的ECOFF.
评估化温度对最小抑制度 (MIC) 值的影响.
为V. parahaemolyticus. AST的国际标准化提供数据.

主要方法:

在八个实验室中采用了标准化汁微稀释协议.
在两个化温度下确定了MIC:35°C (1620小时) 和28°C (2428小时).
数据使用ECOFFinder和正常化电阻解释算法进行分析.

主要成果:

对10种针对V. parahaemolyticus的抗菌剂确定了ECOFF.
塞法胺,佛洛芬尼科尔和三甲/硫甲醇在化温度之间产生了一致的ECOFF.
在两种温度之间观察到恩洛夫洛克萨,甘胺,氧林酸和氧环素的MIC显著变化;56%的配对MIC值是相同的,38%的MIC值不同于一个稀释步骤.

结论:

产生的数据对于建立国际商定的V. parahaemolyticus. ECOFFs至关重要.
化温度可以影响MIC值,因此需要标准化测试条件.
标准化的ECOFF将加强对V. parahaemolyticus.抗菌感应性测试的解释.

关键词:

抗微生物药物抗微生物药物这就是CowT的意思.流行病学界限值是指流行病学界限值.化温度在化温度.在MIC中,MIC是MIC,MIC是MIC.最小的抑制度最小的抑制度这种病毒是Vibrio parahaemolyticus.

更多相关视频

Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates

Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates

Published on: May 30, 2017

Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen

Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen

Published on: June 3, 2018

相关概念视频

Factors Influencing Microbial Growth: Temperature

Factors Influencing Microbial Growth: Temperature

Microorganisms display remarkable adaptations, enabling them to thrive in diverse ecological niches across a wide range of temperatures. Temperature profoundly influences microbial growth by affecting enzymatic activity, membrane fluidity, and other cellular processes.Each microorganism operates within a specific temperature range defined by three cardinal points: minimum, optimum, and maximum. Below the minimum temperature, membranes lose fluidity, halting transport processes. Above the...

Physical Methods for Controlling Microbial Growth: Temperature

Physical Methods for Controlling Microbial Growth: Temperature

Heat is a widely used method to control microbial growth by targeting and denaturing cellular proteins, thereby killing or inactivating microbes. This method's effectiveness is quantified using parameters such as the thermal death point (TDP), thermal death time (TDT), and decimal reduction time (D value). TDP represents the lowest temperature at which all microorganisms in a liquid suspension are eliminated within 10 minutes, whereas TDT is the time necessary to achieve sterilization at a...