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卵细胞成熟期间的炎症降低了发育能力,并增加了芽细胞亡†
Arslan Tariq1, Zachary K Seekford1, John J Bromfield1
1Department of Animal Sciences, University of Florida, 2250 Shealy Drive, Gainesville, FL 32611, United States.
Biology of reproduction
|December 12, 2024
概括
子宫感染会造成炎症性环境,损害牛卵细胞的发育和胚胎质量. 颗粒素细胞衍生因素,而不仅仅是脂聚糖,显著降低发育能力,影响生育结果.
科学领域:
- 生殖生物学 生殖生物学
- 动物科学动物科学
- 细胞和分子生物学是细胞和分子生物学.
背景情况:
- 牛的子宫感染导致卵巢功能障碍和不孕症.
- 细菌内毒素脂聚糖 (LPS) 在子宫感染期间在卵泡液中发现.
- 这种LPS会在粒状细胞中引发炎症反应,从而改变卵细胞的微环境.
研究的目的:
- 研究炎症环境对牛卵细胞发育能力和胚胎质量的影响.
- 为了确定单独的脂聚糖或由粒状细胞在对LPS的反应中产生的因素是否对卵细胞发育受损负责.
主要方法:
- 牛粒状细胞用脂多糖化物 (LPS) 处理,以创建条件介质 (LCM).
- 牛卵细胞在含有LPS,LCM或polymyxin B (LPS隔离剂) 的介质中成熟.
- 受精卵子被培养到囊胚阶段,并评估胚胎质量.
主要成果:
- 在LPS或LCM中成熟显著降低了胚胎细胞的发育和质量,由较少的胚胎体和增加的细胞死亡 (TUNEL阳性细胞) 表明.
- 聚米辛B挽救了LPS诱导的效应,但没有从LCM引起的效应,这表明颗粒状细胞因子是关键因素.
- 这些发现表明,颗粒状细胞产生的炎症媒介会损害卵细胞的能力.
结论:
- 颗粒细胞在对LPS的反应中产生的炎症环境对卵细胞成熟和随后的胚胎发育产生负面影响.
- 颗粒细胞分泌的因子在子宫感染期间危害卵细胞质量方面发挥着关键作用.
- 这项研究突出了导致牛子宫感染导致的不孕症的机制.
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