在溶液和气相中比较部分变质的细胞染色体结构组合,使用交叉连接质谱法
Rebecca L Cain1, Ian K Webb1,2
1Department of Chemistry and Chemical Biology, Indiana University─Indianapolis, Indianapolis, Indiana 46202, United States.
Journal of the American Society for Mass Spectrometry
|December 12, 2024
概括
电子喷雾电离质谱法 (ESI-MS) 显示了溶液和气相中细胞染色体c的类似的变质结构. 分子建模通过疏水膨胀和α螺旋变化确定了展开的启动.
科学领域:
- 生物物理化学 生物物理化学
- 结构生物学 结构生物学
- 质谱测量质量谱测量
背景情况:
- 电喷离子质谱 (ESI-MS) 对于分析完整的蛋白质结构至关重要.
- 在气相离子形成过程中了解蛋白质结构动力学仍然具有挑战性.
- 变质蛋白质结构需要在溶液和气体阶段进行详细的表征.
研究的目的:
- 使用ESI-MS,比较在溶液和气相中的化细胞染色体c的结构.
- 为了确定蛋白质展开过程中结构变化的特定地点.
- 使用分子建模可视化变质蛋白质结构.
主要方法:
- 使用化学交叉连接器探测溶液和气相中的蛋白质结构.
- 在陷电池中使用离子/离子反应来实现气相交联.
- 通过ECD碎片和质谱分析交联产品.
- 执行分子建模与交叉链接数据作为距离限制.
主要成果:
- 对于溶液和气相变质的cytochrome c.观察到相同的交叉链接器识别.
- 溶液和气体相之间的结构差异是最小的.
- 分子建模揭示了由内部疏水扩张启动的展开,随后是α-螺旋变化.
结论:
- 变质的细胞染色体c在溶液和气体阶段都表现出保存的结构.
- 具有交叉链接的ESI-MS提供了对蛋白质展开机制的具体见解.
- 疏水扩张和α螺旋体展开是细胞染色体c变性化的关键早期事件.
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