氨基酸招募到遗传密码中的顺序由最后一个通用共同祖先的蛋白质域解决
Sawsan Wehbi1, Andrew Wheeler1, Benoit Morel2
1Genetics Graduate Interdisciplinary Program, University of Arizona, Tucson, AZ 85721.
概括
遗传代码就是我们的遗传代码.
科学领域:
- 生命的起源 研究 研究 研究
- 分子进化分子进化
- 生物化学 生化学
背景情况:
- 目前关于遗传密码演变的模型依赖于潜在偏见的实验数据.
- 亚生氨基酸的丰富程度可能不准确地反映了早期的生物条件.
- 氨基酸招募到遗传密码的顺序正在争论中.
研究的目的:
- 使用蛋白质域进化推断氨基酸招募到遗传密码的顺序.
- 挑战现有的关于遗传密码历史发展的共识.
- 调查潜在的替代遗传代码,可以追溯到最后一个普世共同祖先 (LUCA) 之前.
主要方法:
- 在最后的普世共同祖先 (LUCA) 中存在的已识别的蛋白质域.
- 重建了LUCA和LUCA前蛋白序列的祖先氨基酸频率.
- 推断的氨基酸招募顺序基于频率偏离控制.
主要成果:
- 较小的氨基酸早些时候被招募到遗传密码中.
- 结合金属的 (氨酸,氨酸) 和含硫的 (氨酸,氨酸) 氨基酸比以前认为的更早被招募.
- 与LUCA序列相比,LUCA前的蛋白质序列显示出不同的氨基酸丰富模式 (芳香,低瓦林/谷氨酸).
结论:
- 氨基酸招募的顺序受到超出简单分子量或非生物可用性因素的影响.
- 氨酸和氨酸的早期可用性支持它们在早期代谢途径 (S-adenosylmethionine) 和蛋白质功能 (金属结合) 中的作用.
- 在LUCA前序列中的丰富模式表明了早期的存在,替代的遗传密码.
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