在牙破中检测Streptococcus mutans和Lactobacilli的分子和微生物检测:对伊朗5-9岁儿童的实验研究
Marzieh Danaei1, Milad Mollaali1,2, Vida Fakharmohialdini1
1Dana Gene Pajoohan Karmania Company, Member of Iran High-Tech Laboratory Network, Kerman, Iran.
Clinical and experimental dental research
|December 12, 2024
概括
这项研究在儿童中发现了乳杆菌和菌株突变菌存在的显著差异.
科学领域:
- 微生物学 微生物学
- 儿科牙科 儿科牙科
- 口腔健康 口腔健康
背景情况:
- 乳球菌和突变性链球菌 (Streptococcus mutans) 都与儿童牙损伤有关.
- 有限的研究同时评估了这些细菌在儿科的定量和定性方面.
研究的目的:
- 在伊朗儿童的牙损伤中检测Streptococcus mutans和Lactobacilli.
- 为了比较斯奈德测试,殖民地计数和PCR的结果.
- 评估细菌水平与病严重程度之间的关联 (dmft指数).
主要方法:
- 在伊朗克尔曼,对120名5-9岁儿童进行了横截面研究.
- 使用殖民地计数 (CFU/ml) 和PCR (16S rRNA和gftB基因) 分析乳酸菌和S. mutans的唾液样本.
- 斯奈德测试用于虫活动评估;使用SPSS 27分析的数据.
主要成果:
- 在dmft指数组中,乳杆菌和S. mutans PCR结果的显著差异.
- 与其他组相比,在dmft=0的儿童中,乳酸菌和S. mutans之间的正相关性计数.
- 斯奈德的测试结果与S. mutans的PCR显著不同,但与Lactobacilli的PCR不同.
结论:
- 这项研究强调了细菌存在,病严重程度和检测方法之间的重要关联.
- 研究结果表明,一个具有成本效益的查计划有潜力,以确定易患牙的儿童.
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