类固醇治疗对儿科急性肝衰竭的影响:TNF-α和miR-12222之间的预后影响和相互作用
Rania M El-Shanawany1, Eman A El-Maadawy2, Hanaa A El-Araby1
1Pediatric Hepatology, Gastroenterology, and Nutrition Department, National Liver Institute, Menoufia University, Menoufia, Shebin El-Koom, 32511, Egypt.
Molecular and cellular pediatrics
|December 12, 2024
概括
在接受类固醇治疗的急性肝衰竭儿童中,较低的miR-122水平与更好的结果相关. 这种微RNA与增加的TNF-α一起,在疾病进展中起作用,并可能有助于预后.
科学领域:
- 儿科胃肠病学 儿科胃肠病学
- 肝病学 肝病学是一种肝病学.
- 分子生物学分子生物学
背景情况:
- 急性肝衰竭 (ALF) 在儿童中具有显著的风险,对类固醇疗效的证据有限.
- 微RNA-122 (miR-122) 是肝功能和疾病的关键参与者,在各种肝脏疾病中表现出改变的表达.
- miR-122被探索为疾病进展,预后和肝病治疗向的潜在生物标志物.
研究的目的:
- 评估小儿ALF患者在类固醇治疗前后的miR-122表达.
- 调查miR-122,瘤亡因子-α (TNF-α) 和ALF治疗反应之间的关系.
- 评估miR-122作为生物标志物的潜力,用于预测儿科ALF的结果.
主要方法:
- 分析了24名患有ALF的儿童的血清样本.
- 使用定量实时RT-PCR (qRT-PCR) 来量化miR-122水平.
- 通过酶相关免疫吸收试验 (ELISA) 测量TNF-α水平.
主要成果:
- 与治疗前和已故患者相比,幸存的ALF儿童在类固醇治疗后显著降低了miR-122水平.
- 与治疗前和已故儿童相比,在接受类固醇治疗后的幸存患者中,TNF-α水平显著升高.
- 在TNF-α和miR-122后类固醇 (r=-0.46,p=0.04) 之间发现了负相关性,miR-122在区分幸存者中显示了72%的敏感性和67%的特异性.
结论:
- 在接受类固醇治疗的儿科ALF患者中,较低的miR-122水平与改善的结果有关.
- 虽然不是一个独立的标志物,但miR-122可能作为ALF生物标志物小组的一部分有价值.
- 升高的TNF-α和降低的miR-122表明它们参与ALF病理生理学,需要进一步调查.
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