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基于aptamer和SYBR Green I的光检测鱼类中的马拉绿色

Jia Wang¹, Chengyi Hong¹, Zhengzhong Lin¹

¹College of Ocean Food and Biological Engineering, Jimei University, Xiamen, China.

Journal of food and drug analysis

|December 12, 2024

概括

一种新的光方法可以准确地检测鱼类中的马拉绿 (MG). 这种敏感的技术使用MG-aptamer和SYBR Green I,提供比国家和欧洲标准更低的检测极限.

科学领域:

分析化学分析化学
生物化学生化学

背景情况:

马拉绿 (MG) 是一种用于水产养殖的合成染料,但其残留物会对健康造成风险.
准确和灵敏的MG检测方法对于食品安全至关重要.

研究的目的:

开发一种简单的定量光方法,用于检测马拉绿 (MG).
建立一种高灵敏度和准确度的方法,用于在鱼样中检测MG.

主要方法:

使用了马拉绿特异性体 (Apt) 和色SYBR绿I (SGI) 的调整开关效应.
在MG与Apt-SGI复合体结合时监控光火.
为光检测优化反应条件.

主要成果:

证明了光火效率与MG度从0.02到1.0μmol L-1.1之间的线性关系.
达到0.67μg kg-1的检测极限,超过了国家和欧洲标准.
获得高恢复率 (87.1%106.9%),相对标准偏差较低 (<6.4%).
与高性能液态染色学 (HPLC) 相比验证的结果.

结论:

开发的光方法提供了一种快速,准确和敏感的方法来检测鱼类中的马拉绿色.
该方法符合严格的监管要求,可用于日常食品安全监测.

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