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对子特定的肉量化类标记物的LC-QTOF-MS评估

Anna Stachniuk¹, Anna Kozub¹, Renata Czeczko²

¹Department of Bioanalytics, Medical University of Lublin, ul. Jaczewskiego 8b, 20-090, Lublin, Poland.

Journal of food and drug analysis

|December 12, 2024

概括

这项研究开发了一种质谱法,以准确量化混合物中的子和肉含量. 经过验证的标记物为可靠的肉类真实性和可追溯性提供了基础.

科学领域:

食品科学食品科学食品科学
分析化学分析化学
生物技术是生物技术.

背景情况:

准确量化肉类物种对于食品的真实性和合规性至关重要.
现有的肉类物种识别和量化方法可能很复杂,需要专门的专业知识.

研究的目的:

评估标记物,以准确量化各种肉类混合物中的子和肉.
为了确定选定的标记物的线性,检测极限和量化.
为开发无标签,绝对量化方法提供基础,用于肉类物种.

主要方法:

使用液体染色学-四极飞行时间质谱法 (LC-QTOF-MS) 来分析信号.
准备并分析了二元和三元肉混合物,其中子和肉的百分比不同.
确定了子和的特异性三性标记物,并评估了它们的质量信号的线性.

主要成果:

在11种肉上有5种,在12种肉中有5种表现出良好的线性 (R2 > 0.97).
该方法实现了0.43-1.91% [w/w]之间的检测极限和1.44-6.38% [w/w]之间的量化极限.
在多元成分混合物中,精确确定了子和肉的百分比.

结论:

开发的LC-QTOF-MS方法使用选定的标记物是有效量化子和肉含量.
这些发现支持开发强大的,无标签的量化技术来验证肉类的真实性.
经过验证的标记物作为未来食品分析和安全研究的宝贵资源.

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