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特尔皮宁-4-ol对人类角膜上皮质的影响

Joo-Hee Park¹, Choul Yong Park²

¹Department of Ophthalmology, Dongguk University Ilsan Hospital, Goyang, Republic of Korea.

Translational vision science & technology

|December 12, 2024

概括

特尔皮宁-4-ol (T4O) 在人角膜上皮细胞 (HCEC) 中引起显著的毒性,这种毒性取决于剂量和时间. 这种毒性与活性氧物种 (ROS) 增加和细胞生存途径受损有关.

科学领域:

眼科医生眼科眼科
毒理学毒理学毒理学
细胞生物学细胞生物学

背景情况:

-4- (T4O) 是茶树油的主要成分.
茶树油用于各种化品和治疗产品.
对于T4O的眼睛毒性,需要进行彻底的调查,以确保安全的应用.

研究的目的:

调查特皮宁-4-ol (T4O) 对初级培养的人类角膜上皮细胞 (HCEC) 的毒性.

主要方法:

在不同时间内,HCEC暴露于不同度的T4O (0%-0.1%).
用CCK8试验和细胞计数来评估细胞活力.
分析了细胞内活性氧物种 (ROS),细胞形态,活性丝和关键蛋白质表达 (mTOR,Akt,Bcl-xL,BAX).

主要成果:

在HCEC中,T4O诱导了剂量依赖性毒性,即使在低度和短时间暴露时也观察到显著的生存能力降低.
长时间暴露于T4O增强了细胞毒性,导致更高度的细胞完全死亡.
毒性与细胞内ROS增加,细胞存活途径活性降低 (p-mTOR,p-Akt,Bcl-xL),BAX表达增加和细胞粘附改变有关.

结论:

暴露于T4O会在HCEC中引起显著的毒性,受度和潜伏时间的影响.
毒性机制涉及氧化应激和破坏细胞生存信号通路.
这些发现为T4O在眼科应用中的安全使用提供了关键数据,例如针对Demodex感染的眼擦拭剂.

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