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使用在线SPE-UHPLC-HRMS在人血清中精确和精确地确定32个PFAS

Masho Hilawie Belay¹, Elisa Robotti², Arianna Ghignone²

¹Department of Sciences and Technological Innovation, University of Piemonte Orientale, Viale Teresa Michel 11, 15121 Alessandria, Italy; Department of Chemistry, Mekelle University, P. O. Box 231, Mekelle, Ethiopia.

Journal of hazardous materials

|December 12, 2024

概括

一种新方法准确量化了人类血清中的32种持久性per-和多基物质 (PFAS). 这种高度敏感的技术提高了检测极限,有助于生物监测和了解PFAS的健康风险.

科学领域:

环境化学环境化学
分析化学分析化学
毒理学毒理学毒理学

背景情况:

和多醇基物质 (PFAS) 是持久性环境污染物,与人类健康有害影响有关,包括增加癌症风险.
在生物矩阵中精确量化广泛的PFAS对于人类健康风险评估和生物监测至关重要.

研究的目的:

开发和验证一种高度敏感的分析方法,用于量化人血清中32种遗留和新兴的PFAS.
建立一种适用于高通量生物监测研究的可靠方法.

主要方法:

在线固相提取 (SPE) 与超高性能液体染色学-高分辨率质谱学 (UHPLC-HRMS) 相结合.
使用各种溶剂组合和主要成分分析优化蛋白质沉和颗粒冲洗步骤.
根据ISO/IEC 17025标准进行验证,包括对线性,精度和准确性的评估.

主要成果:

获得的量化极限比以前的方法 (8.9到27 ng/L) 低5到15倍.
证明了出色的线性 (R2 = 0.977-0.997),强大的精度 (RSDs 1.3-14.0%),和准确性 (72.7-106%的恢复).
成功地将该方法应用于人体血清样本,使其能够同时确定各种PFAS,包括以太原体.

结论:

在线开发的SPE-UHPLC-HRMS方法为人类血清中PFAS分析提供了前所未有的灵敏度和广泛的范围.
这种经过验证的方法适用于高通量生物监测,推进PFAS暴露和健康影响的研究.
该研究引入了第一个在线SPE方法,用于同时确定广泛的PFAS,包括新型以太类同源.

关键词:

高分辨率的质谱仪.人类生物监测人类生物监测方法开发方法的开发方法.在PFAS中,有很多方法.血清的预处理

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