一项关于狼稳定纳米载体对黄素口服生物利用性的体外研究,该研究对黄素进行了稳定
Negin Hashemi1, Emmanouil Tsochatzis1, Martin Krøyer Rasmussen1
1Department of Food Science, Aarhus University, Agro Food Park 48, 8200 Aarhus N, Denmark.
Food chemistry
|December 12, 2024
概括
卢平蛋白纳米颗粒显著改善了黄素.
科学领域:
- 食品科学与技术 食品科学与技术
- 营养科学 营养科学
- 生物材料科学 生物材料科学
背景情况:
- 黄素是一种生物活性化合物,具有较差的生物可用性.
- 开发有效的输送系统对于增强黄素对健康的益处至关重要.
- 狼蛋白分离物为封装生物活性化合物提供了一个有前途的自然基质.
研究的目的:
- 评估包装在基于狼蛋白分离物的载体中的黄素的生物可访问性和生物可用性.
- 为了比较狼蛋白纳米颗粒与乳液对黄素稳定性的保护作用.
- 为了研究黄素在体外消化和Caco-2/HT29-MTX细胞吸收过程中的命运.
主要方法:
- 将一个体外胃肠道消化 (INFOGEST) 模型与一个体外共同培养吸收模型 (Caco-2/HT29-MTX) 结合起来.
- 使用目标超高性能四极飞行时间质谱法 (UHPLC-QTOF-MS) 来定量黄素.
- 分析黄素回收在顶端,细胞和底侧分数.
主要成果:
- 狼蛋白纳米颗粒显示,与O/W乳液相比,黄素的保护优于黄素,70%的恢复率与≤35%相比.
- 黄素主要存在于纳米颗粒消化器中的不溶性阶段,与乳液中的状阶段形成鲜明对比.
- 显著的黄素被保留在细胞单层中 (54%的纳米颗粒,≤24%的乳液),在底侧部分检测到代谢物.
结论:
- 狼蛋白纳米颗粒通过保护它免受降解来增强黄素的生物可访问性.
- 输送系统的结构显著影响封装生物活性化合物的生物可访问性.
- 对食物矩阵效应的进一步研究对于优化从食物来源输送生物活性化合物至关重要.
相关概念视频
Methods for Studying Drug Absorption: In vitro
202
In vitro experiments are crucial for understanding the transport and absorption of drugs through biological materials. These studies employ varied methods such as the diffusion cell method, the everted sac technique, and the everted ring technique.
The diffusion cell method uses a two-compartment cell, including a donor compartment with the drug solution, which simulates the environment where the drug is applied, and a receptor compartment with a buffer solution, which simulates the environment...
The diffusion cell method uses a two-compartment cell, including a donor compartment with the drug solution, which simulates the environment where the drug is applied, and a receptor compartment with a buffer solution, which simulates the environment...
202
Methods for Studying Drug Absorption: In situ
202
In situ experiments, such as the Doluisio method and Single-Pass Perfusion technique, provide critical insights into drug uptake by simulating in vivo conditions for drug absorption.
The Doluisio method involves perfusing a prepared segment of a rat's small intestine with a solution of radiolabeled drug and a non-absorbable marker. This helps to differentiate between absorbed and non-absorbed drug concentrations. The intestinal segment is connected at both ends using tubing and syringes,...
The Doluisio method involves perfusing a prepared segment of a rat's small intestine with a solution of radiolabeled drug and a non-absorbable marker. This helps to differentiate between absorbed and non-absorbed drug concentrations. The intestinal segment is connected at both ends using tubing and syringes,...
202
Factors Affecting Dissolution: Drug Permeability, Stability and Stereochemistry
174
Orally administered drugs primarily enter the systemic circulation via passive diffusion through the intestinal membranes. The drug's absorption is influenced by drug stability in the gastrointestinal GI tract, membrane permeability, the surface area available for absorption, luminal drug concentration, and residence time in the lumen. Drug permeability can be enhanced by adjusting the lipophilicity, polarity, or molecular size of the drug, promoting its passive transport across intestinal...
174
Factors Influencing Bioavailability: First-Pass Elimination
6.1K
When a drug is taken orally, it undergoes a journey starting from the gastrointestinal (GI) tract, passing through the portal vein, reaching the liver, and finally entering the systemic circulation. This process involves the absorption of the drug across the GI tract. The liver is the primary site for metabolizing the drug, with some metabolism also occurring in the gut wall. This journey significantly reduces the quantity of the drug that reaches the systemic circulation, a phenomenon known as...
6.1K
Bioavailability: Overview
2.7K
Bioavailability refers to the proportion of an unaltered drug that, after administration, enters the systemic circulation and can be distributed to the desired action site. Factors such as gastrointestinal (GI) absorption and liver biotransformation influence the bioavailability of a drug when it is administered orally. When a drug is administered intravenously, it enters the systemic circulation directly; by definition, its bioavailability is assumed to be 100%. The bioavailability of an...
2.7K
Factors Affecting Dissolution: Particle Size and Effective Surface Area
727
Dissolution kinetics, an essential aspect of oral drug delivery, is significantly influenced by the drug's particle size. According to the Noyes-Whitney dissolution model, the dissolution rate correlates directly with the drug's surface area. The larger the surface area, the higher the drug's solubility in water, leading to a faster drug dissolution rate. Reducing particle size increases the effective surface area, enhancing the dissolution process. Micronization and nanosizing are...
727


