氨基功能化的纤维素珠支持laccase:一个双功能的催化剂,用于同时吸附和酶转化四环素
Sucheng Liu1, Bang An1, Yaoyao Wang1
1School of Chemical Engineering and Pharmacy, Wuhan Institute of Technology, LiuFang Campus, No.206, Guanggu 1st road, Donghu New & High Technology Development Zone, Wuhan 430205, Hubei Province, PR China.
International journal of biological macromolecules
|December 12, 2024
概括
研究人员开发了氨基功能化的纤维素珠 (ACB) 来固定乳糖,以有效地从水中去除四环素 (TC). 这种新型酶催化剂通过同时吸附和酶降解实现了82%的TC去除.
科学领域:
- 生物技术是生物技术.
- 环境科学 环境科学
- 材料科学 材料科学 材料科学
背景情况:
- 水中环素 (TC) 污染对环境和健康构成风险.
- 酶固定对于开发稳定且可重复使用的生物催化剂至关重要.
- 纤维素衍生材料为酶支持提供可持续和多功能平台.
研究的目的:
- 开发一种新型的纤维素衍生的支,用于拉卡斯固定.
- 为了研究将静止乳糖酶用于从水中去除四环素的应用.
- 描述固定酶催化剂的特征并评估其性能.
主要方法:
- 氨基功能化的纤维素珠 (ACBs) 由纤维素珠 (CBs) 和聚乙烯胺通过甲交联合成.
- 通过希夫基反应,Laccase被协同固定在ACB上,形成Lac-ACBs.
- 使用SEM-EDS,FT-IR,XRD,BET和EA进行了ACB和Lac-ACB的表征.
- 使用Lac-ACBs进行了对四环素的同时吸附和酶降解.
主要成果:
- 开发的Lac-ACBs表现出卓越的稳定性和可重复使用性,在15次催化周期后保持72%的活性.
- 使用Lac-ACBs,从水中获得82%的四环素去除效率.
- 鉴定证实了酶催化剂的成功固定和结构完整性.
- 对转化产品的分析显示氧化,脱和脱甲基化是关键的降解途径.
结论:
- 新型氨基功能化纤维素珠 (ACBs) 提供了有效的支持,以使乳酶不动.
- 拉克-ACB具有很高的稳定性,可重复使用性和在从水溶液中去除四环素的效率.
- 同时吸附和酶降解过程为水的整治提供了一个有前途的方法.
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