空间染色体的组织和适应的耐辐射极端生物Deinococcus radiodurans的空间染色体
Qin-Tian Qiu1, Cai-Yun Zhang1, Zhi-Peng Gao1
1Hubei Key Laboratory of Agricultural Bioinformatics, College of Informatics, Huazhong Agricultural University, Wuhan, China.
The Journal of biological chemistry
|December 12, 2024
概括
这项研究揭示了抗辐射的Deinococcus radiodurans在紫外线 (UV) 照射后如何重组其3D基因组结构. 紫外线暴露会改变染色体相互作用域,影响这种极端爱好者的基因表达和生存机制.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 生物物理学的生物物理.
背景情况:
- 迪诺科克 (Deinococcus radiodurans) 是研究抗压力的极端动物模型.
- 其显著的DNA修复和抗辐射能力是众所周知的.
- 然而,其基因组的空间组织及其对压力的反应尚不清楚.
研究的目的:
- 为了研究Deinococcus radiodurans的3D基因组结构.
- 在紫外线 (UV) 照射后分析染色体构造的变化.
- 了解基因组组织在细菌应激反应中的作用.
主要方法:
- 使用了染色体形状捕获 (3C) 技术.
- 转录组数据分析与3C数据相结合.
- 评估了染色体相互作用域 (CID) 和基因表达的变化.
主要成果:
- 紫外线照射导致了染色体短距离相互作用的减少.
- 较小的CID在紫外线暴露时合并为较大的CID.
- 上调基因经常位于特定的CID边界附近,核相关蛋白DreEbfC被确定为关键调节者.
结论:
- 这项研究揭示了D. radiodurans在正常和紫外线照射条件下的3D基因组结构.
- 染色体构造的变化与基因表达变化和生存策略有关.
- DrEbfC在通过染色体结构调节代谢途径方面发挥着至关重要的作用,在压力期间影响细菌的生理状态.
关键词:
迪诺科克斯 (Deinococcus radiodurans) 是一种可怕的植物.细菌遗传学 细菌遗传学染色体重塑 染色体重塑的方法染色体组织是染色体组织.能量代谢 能量代谢基因组结构 基因组结构核相关蛋白质是一种核相关蛋白质.转录法规 转录法规 转录法规紫外线辐射辐射的紫外线辐射.更多相关视频
08:11Author Spotlight: Understanding Microbe Adaptation Using Innovative Techniques for Exploring Thermophilic Evolution
Published on: June 14, 2024
670
11:45Fluorescence Live-cell Imaging of the Complete Vegetative Cell Cycle of the Slow-growing Social Bacterium Myxococcus xanthus
Published on: June 20, 2018
9.5K
相关概念视频
Nucleoid
1
The nucleoid represents a structurally and functionally distinct region within prokaryotic cells, where the cell's DNA and associated proteins are housed. Unlike eukaryotic cells, prokaryotes lack a membrane-bound nucleus, and the nucleoid facilitates the organization and accessibility of the genetic material within this constraint. The DNA in most bacteria and archaea exists as a single, circular, double-stranded molecule that is highly compacted through supercoiling and interactions with...
1
Polytene Chromosomes
9.9K
Polytene chromosomes are giant interphase chromosomes with several DNA strands placed side by side. They were discovered in the year 1881 by Balbiani in salivary glands, intestine, muscles, malpighian tubules, and hypoderm of larvae Chironomus plumosus. Hence, these are also called "Salivary gland chromosomes." These are found in insects of the order Diptera and Collembola; in certain organs of mammals; and synergids, antipodes of flowering plants. Polytene chromosomes are also...
9.9K
Chromosome Structure
22.6K
A functional eukaryotic chromosome must contain three elements: a centromere, telomeres, and numerous origins of replication.
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...
22.6K
Genomic DNA in Prokaryotes
43.4K
The genome of most prokaryotic organisms consists of double-stranded DNA organized into one circular chromosome in a region of cytoplasm called the nucleoid. The chromosome is tightly wound, or supercoiled, for efficient storage. Prokaryotes also contain other circular pieces of DNA called plasmids. These plasmids are smaller than the chromosome and often carry genes that confer adaptive functions, such as antibiotic resistance.
Genomic Diversity in Bacteria
Although bacterial genomes are much...
Genomic Diversity in Bacteria
Although bacterial genomes are much...
43.4K
Lampbrush Chromosomes
7.8K
In 1882, Flemming observed lampbrush chromosomes (LBC) in salamander eggs. Later in 1892, Rückert observed LBCs in shark egg cells and coined the term "lampbrush chromosomes" because they looked like brushes used to clean kerosene lamps.
LBCs are made up of two pairs of conjugating homologous chromatids. Each chromatid consists of alternatively positioned regions of condensed-inactive chromatin and loosely placed-active side loops, which can be contracted and extended. The loops...
LBCs are made up of two pairs of conjugating homologous chromatids. Each chromatid consists of alternatively positioned regions of condensed-inactive chromatin and loosely placed-active side loops, which can be contracted and extended. The loops...
7.8K
Karyotyping
57.8K
Overview
57.8K
